Development of taxon-specific sequence characterized amplified region (SCAR) markers based on actin sequences and DNA amplification fingerprinting (DAF): a case study in the Phoma exigua species complex. (Articolo in rivista)

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  • Development of taxon-specific sequence characterized amplified region (SCAR) markers based on actin sequences and DNA amplification fingerprinting (DAF): a case study in the Phoma exigua species complex. (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • Aveskamp M.M., Woudenberg J.H.C., De Gruyter J., Turco E., Groenewald J.Z., Crous P.W. (2009)
    Development of taxon-specific sequence characterized amplified region (SCAR) markers based on actin sequences and DNA amplification fingerprinting (DAF): a case study in the Phoma exigua species complex.
    in Molecular plant pathology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Aveskamp M.M., Woudenberg J.H.C., De Gruyter J., Turco E., Groenewald J.Z., Crous P.W. (literal)
Pagina inizio
  • 403 (literal)
Pagina fine
  • 414 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 10 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • - Aveskamp M.M., Woudenberg J.H.C., De Gruyter J., Groenewald J.Z., CBS Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands, and Dutch Plant Protection Service (PD), Geertjesweg 15, 6706 EA Wageningen, The Netherlands -Turco E., - CNR, Istituto per la Protezione delle Piante, Sezione di Firenze, Via Madonna del Piano 10, 50019 Sesto Fiorentino (FI), Italia -Crous P.W., Wageningen University and Research Centre (WUR), Laboratory of Phytopathology, Binnenhaven 5, 6709 PD Wageningen, The Netherlands (literal)
Titolo
  • Development of taxon-specific sequence characterized amplified region (SCAR) markers based on actin sequences and DNA amplification fingerprinting (DAF): a case study in the Phoma exigua species complex. (literal)
Abstract
  • Phoma exigua is considered to be an assemblage of at least nine varieties that are mainly distinguished on the basis of host specificity and pathogenicity. However, these varieties are also reported to be weak pathogens and secondary invaders on non-host tissue. In practice, it is difficult to distinguish P. exigua from its close relatives and to correctly identify isolates up to the variety level, because of their low genetic variation and high morphological similarity. Because of quarantine issues and phytosanitary measures, a robust DNA-based tool is required for accurate and rapid identification of the separate taxa in this species complex. The present study therefore aims to develop such a tool based on unique nucleotide sequence identifiers. More than 60 strains of P. exigua and related species were compared in terms of partial actin gene sequences, or analysed using DNA amplification fingerprinting (DAF) with short, arbitrary, mini-hairpin primers. Fragments in the fingerprint unique to a single taxon were identified, purified and sequenced. Alignment of the sequence data and subsequent primer trials led to the identification of taxon-specific sequence characterized amplified regions (SCARs), and to a set of specific oligonucleotide combinations that can be used to identify these organisms in plant quarantine inspections. (literal)
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