http://www.cnr.it/ontology/cnr/individuo/prodotto/ID40566
Response of the carotenoidless mutant Rhodobacter sphaeroides growing cells to cobalt and nickel exposure (Articolo in rivista)
- Type
- Label
- Response of the carotenoidless mutant Rhodobacter sphaeroides growing cells to cobalt and nickel exposure (Articolo in rivista) (literal)
- Anno
- 2009-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.ibiod.2009.05.001 (literal)
- Alternative label
Italiano, F.; Buccolieri, A.; Giotta, L.; Agostiano, A.; Valli, L.; Milano, F.; Trotta, M. (2009)
Response of the carotenoidless mutant Rhodobacter sphaeroides growing cells to cobalt and nickel exposure
in International biodeterioration & biodegradation
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Italiano, F.; Buccolieri, A.; Giotta, L.; Agostiano, A.; Valli, L.; Milano, F.; Trotta, M. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
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- Scopu (literal)
- ISI Web of Science (WOS) (literal)
- Google Scholar (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- CNR, Istituto per i Processi Chimico-Fisici, Sezione di Bari
Dipartimento di Scienza dei Materiali, Università del Salento
Dipartimento di Chimica, Università degli Studi di Bari
Dipartimento di Ingegneria dell'Innovazione, Università del Salento (literal)
- Titolo
- Response of the carotenoidless mutant Rhodobacter sphaeroides growing cells to cobalt and nickel exposure (literal)
- Abstract
- The interaction of cobalt (Co(2+)) and nickel (Ni(2+)) ions with whole cells of the photosynthetic purple bacterium Rhodobacter sphaeroides strain R26 was investigated. Active and passive uptakes were examined in cells grown in the presence of increasing amounts of Co(2+) and Ni(2+). Inductively coupled plasma atomic emission spectroscopy (ICP-AES), pH titration, and attenuated total reflection Fourier transform infrared (ATR-FTIR) spectroscopy were used to assess the role of cell envelope and metabolism in accumulating the two heavy metals. The chosen microorganism was able to uptake cobalt and nickel up to 2.2 and 0.25 mg per gram of dried cells respectively, with the largest part found bound to the cell surface. Carboxylate groups lying on the cell wall of this Gram-negative bacterium proved to be the major candidates for binding protons and metal cations. Co(2+) was found to interfere with Mg(2+) extracellular immobilization and transport across the membrane, indicating that these ions share binding sites on the cell envelope and ion transport systems. According to the presence of a competition mechanism, bacterial growth experiments showed that high Mg(2+) concentrations are able to rescue R. sphaeroides from Co(2+) toxicity. (C) 2009 Elsevier Ltd. All rights reserved. (literal)
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