http://www.cnr.it/ontology/cnr/individuo/prodotto/ID39390
Determination of hydrogen sulfide and volatile thiols in air samples by mercury probe derivatization coupled with liquid chromatographyatomic fluorescence spectrometry (Articolo in rivista)
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- Label
- Determination of hydrogen sulfide and volatile thiols in air samples by mercury probe derivatization coupled with liquid chromatographyatomic fluorescence spectrometry (Articolo in rivista) (literal)
- Anno
- 2006-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.aca.2006.07.004 (literal)
- Alternative label
Emilia Bramanti, Lucia D'Ulivo, Cristina Lomonte, Massimo Onor, Roberto Zamboni, Giorgio Raspi, Alessandro D'Ulivo. (2006)
Determination of hydrogen sulfide and volatile thiols in air samples by mercury probe derivatization coupled with liquid chromatographyatomic fluorescence spectrometry
in Analytica chimica acta (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- Emilia Bramanti, Lucia D'Ulivo, Cristina Lomonte, Massimo Onor, Roberto Zamboni, Giorgio Raspi, Alessandro D'Ulivo. (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
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- Scopu (literal)
- PubMe (literal)
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- a Italian National Research Council, CNR-Istituto per i Processi Chimico-Fisici, Laboratory of Instrumental Analytical Chemistry,
Via G. Moruzzi 1, 56124 Pisa, Italy
b Ambiente s.c.r.l., Via Frassina 21, 54033, Carrrara, Massa, Italy
c Department of Chemistry and Industrial Chemistry, University of Pisa, Via Risorgimento 35, 56126 Pisa, Italy (literal)
- Titolo
- Determination of hydrogen sulfide and volatile thiols in air samples by mercury probe derivatization coupled with liquid chromatographyatomic fluorescence spectrometry (literal)
- Abstract
- A new procedure is proposed for the sampling and storage of hydrogen sulphide (H2S) and volatile thiols (methanethiol or methyl mercaptan,
ethanethiol and propanethiol) for their determination by liquid chromatography. The sampling procedure is based on the trapping/pre-concentration
of the analytes in alkaline aqueous solution containing an organic mercurial probe p-hydroxymercurybenzoate, HO-Hg-C6H4-COO- (PHMB),
where they are derivatized to stable PHMB complexes based on mercury-sulfur covalent bonds. PHMB complexes are separated on a C18 reverse
phase column, allowing their determination by liquid chromatography coupled with sequential non-selective UV-vis (DAD) and mercury specific
(chemical vapor generation atomic fluorescence spectrometry, CVGAFS) on-line detectors. PHMB complexes, S(PHMB)2CH3S-PHMB, C2H5SPHMB
and C3H7S-PHMB, are stable alt least for 12 h at room temperature and for 3 months if stored frozen (-20 oC).
The best analytical figures of merits in the optimized conditions were obtained by CVGAFS detection, with detection limits (LODc) of 9.7 ?gL-1
forH2S, 13.7 ?gL-1 forCH3SH, 17.7 ?gL-1 forC2H5SHand 21.7 ?gL-1 forC3H7SHin the trapping solution in form ofRS-PHMBcomplexes, the
relative standard deviation (R.S.D.) ranging between 1.0 and 1.5%, and a linear dynamic range (LDR) between 10 and 9700 ?gL-1. Conventional
UV absorbance detectors tuned at 254 nm can be employed as well with comparable R.S.D. and LDR, but with LODc one order of magnitude
higher than AFS detector and lower specificity. The sampling procedure followed by LC-DAD-CVGAFS analysis has been validated, as example,
for H2S determination by a certified gas permeation tube as a source of 3.071±0.154?g min-1 of H2S, giving a recovery of 99.8±7% and it has
been applied to the determination of sulfur compounds in real gas samples (biogas and the air of a plant for fractional distillation of crude oil).
© 2006 Elsevier B.V. All rights reserved. (literal)
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