Hydrogel for cell housing in the brain and in the spinal cord. (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Hydrogel for cell housing in the brain and in the spinal cord. (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.5301/IJAO.2011.6488 (literal)

Alternative label

• Giuseppe Perale1, Carmen Giordano1, Fabio Bianco2, Filippo Rossi1, Marta Tunesi1, Francesco Daniele1, Filippo Crivelli3, Michela Matteoli4, Maurizio Masi1 (2011)
  Hydrogel for cell housing in the brain and in the spinal cord.
  in The International Journal of Artificial Organs (Testo stamp.)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Giuseppe Perale1, Carmen Giordano1, Fabio Bianco2, Filippo Rossi1, Marta Tunesi1, Francesco Daniele1, Filippo Crivelli3, Michela Matteoli4, Maurizio Masi1 (literal)

Pagina inizio

• 295 (literal)

Pagina fine

• 303 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 34 (literal)

Rivista

• ?The ?International Journal of Artificial Organs (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1Department of Chemistry, Materials and Chemical Engineering, Polytechnic University of Milan, Milan - Italy 2Neuro-Zone S.r.l., Segrate, Milan - Italy 3Pathological Anatomy Department, Sant'Antonio Abate di Gallarate Hospital, Gallarate (Milan) - Italy 4Department of Medical Pharmacology, University of Milan, Milan - Italy (literal)

Titolo

• Hydrogel for cell housing in the brain and in the spinal cord. (literal)

Abstract

• Purpose: Neurons in the adult mammalian central nervous system do not proliferate or renew themselves and consequently strong interest in cell replacement therapies to repair brain and spinal cord damages has emerged in the last decade. ?Methods: An injectable resorbable hydrogel with a controlled nanostructure, specifically designed for neural cell housing, was developed together with a new protocol for building three-dimensional (3D) biohybrid cell/hydrogel constructs: cells are housed within the polymeric matrix which is directly built with a specific cell culture media. This matrix was tested with standard glial populations, primary astrocytes and mesenchymal stem cells.?Results: Physico-chemical characterization of the hydrogel matrix confirmed a 2- week (+ 2 days) stability before massive degradation; mean mesh size of about 5 nm and thixotropic behavior with transition yield stress at 60+5 Pa. Cell survival within the hydrogel resulted in about 55±5% (minimum value) survivals, data also confirmed by optical assessments. Cell viability also remained high after extraction from the gel, indicating survival to inclusion latency period. ?Conclusions: Since the intimate structure of the gel mimics extracellular matrix cells as would be expected to be found in an in vivo context, this polymeric formulation is a promising base for building 3D constructs for neural cell housing, in which cells are embedded and kept alive directly from the time of polycondensation. (literal)

Prodotto di

• Farmacologia Cellulare e Molecolare delle Cellule Nervose (ME.P02.007.001) (Modulo)
• Institute of neuroscience (IN) (Istituto)

Autore CNR

• MICHELA MATTEOLI (Unità di personale esterno)

Incoming links:

Prodotto

• Institute of neuroscience (IN) (Istituto)
• Farmacologia Cellulare e Molecolare delle Cellule Nervose (ME.P02.007.001) (Modulo)

Autore CNR di

• MICHELA MATTEOLI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• ?The ?International Journal of Artificial Organs (Rivista)