A comparative study of the effects of the intravenous self-administration or subcutaneous minipump infusion of nicotine on the expression of brain neuronal nicotinic receptor subtypes. (Articolo in rivista)

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Label
  • A comparative study of the effects of the intravenous self-administration or subcutaneous minipump infusion of nicotine on the expression of brain neuronal nicotinic receptor subtypes. (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1124/mol.110.064071 (literal)
Alternative label
  • Moretti M (1), Mugnaini M(2), Tessari M (2), Zoli M (3), Gaimarri A(1), Manfredi I (1), Pistillo F(1), Clementi F(1) and Gotti C(1) (2010)
    A comparative study of the effects of the intravenous self-administration or subcutaneous minipump infusion of nicotine on the expression of brain neuronal nicotinic receptor subtypes.
    in Molecular pharmacology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Moretti M (1), Mugnaini M(2), Tessari M (2), Zoli M (3), Gaimarri A(1), Manfredi I (1), Pistillo F(1), Clementi F(1) and Gotti C(1) (literal)
Pagina inizio
  • 287 (literal)
Pagina fine
  • 296 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 78 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. Univ Milan, Dept Med Pharmacol, CNR, Neurosci Inst Milano, I-20133 Milan, Italy 2. GlaxoSmithKline Med Res Ctr, Neurosci Ctr Excellence Drug Discovery, Addict & Sleep Disorders Discovery Performance Un, Dept Biol, Verona, Italy 3. Univ Modena & Reggio Emilia, Physiol Sect, Dept Biomed Sci, Modena, Italy (literal)
Titolo
  • A comparative study of the effects of the intravenous self-administration or subcutaneous minipump infusion of nicotine on the expression of brain neuronal nicotinic receptor subtypes. (literal)
Abstract
  • Long-term nicotine exposure changes neuronal acetylcholine nicotinic receptor (nAChR) subtype expression in the brains of smokers and experimental animals. The aim of this study was to investigate nicotine-induced changes in nAChR expression in two models commonly used to describe the effects of nicotine in animals: operant (two-lever presses) intravenous self-administration (SA) and passive subcutaneous nicotine administration via an osmotic minipump (MP). In the MP group, alpha 4 beta 2 nAChRs were up-regulated in all brain regions, alpha 6 beta 2* nAChRs were down-regulated in the nucleus accumbens (NAc) and caudate-putamen, and alpha 7 nAChRs were up-regulated in the caudal cerebral cortex (CCx); the up-regulation of alpha 4 beta 2 alpha 5 nAChRs in the CCx was also suggested. In the SA group, alpha 4 beta 2 up-regulation was lower and limited to the CCx and NAc; there were no detectable changes in alpha 6 beta 2* or alpha 7 nACRs. In the CCx of the MP rats, there was a close correlation between the increase in alpha 4 beta 2 binding and alpha 4 and beta 2 subunit levels measured by means of Western blotting, demonstrating that the up-regulation was due to an increase in alpha 4 beta 2 proteins. Western blotting also showed that the increase in the alpha 2 subunit exceeded that of the alpha 4 subunit, suggesting that a change in alpha 4 beta 2 stoichiometry may occur in vivo as has been shown in vitro. These results show that nicotine has an area-specific effect on receptor subtypes, regardless of its administration route, but the effect is quantitatively greater in the case of MP administration (literal)
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