Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones. (Articolo in rivista)

Type
Label
  • Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones. (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Alternative label
  • Bruzzone S, Verderio C, Schenk U, Fedele E, Zocchi E, Matteoli M, De Flora A (2004)
    Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones.
    in Journal of neurochemistry
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bruzzone S, Verderio C, Schenk U, Fedele E, Zocchi E, Matteoli M, De Flora A (literal)
Pagina inizio
  • 264 (literal)
Pagina fine
  • 272 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 89 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1. Univ Genoa, Dept Expt Med, Biochem Sect, I-16132 Genoa, Italy 2. Univ Genoa, Dept Expt Med, Pharmacol Sect, I-16132 Genoa, Italy 3. Univ Genoa, MIUR Ctr Excellence Biomed Res, I-16132 Genoa, Italy 4. Univ Milan, CNR, Inst Neurosci, MIUR Ctr Excellence Neurodegenerat Dis, I-20122 Milan, Italy 5. Univ Milan, Dept Med Pharmacol, I-20122 Milan, Italy (literal)
Titolo
  • Glutamate-mediated overexpression of CD38 in astrocytes cultured with neurones. (literal)
Abstract
  • Recently, a new system of astrocyte-neurone glutamatergic signalling has been identified. It is started in astrocytes by ectocellular, CD38-catalysed conversion of NAD(+) to the calcium mobilizer cyclic ADP-ribose (cADPR). This is then pumped by CD38 itself into the cytosol where the resulting free intracellular Ca2+ concentration [Ca2+](i) transients elicit an increased release of glutamate, which can induce an enhanced Ca2+ response in neighbouring neurones. Here, we demonstrate that co-culture of either cortical or hippocampal astrocytes with neurones results in a significant overexpression of astrocyte CD38 both on the plasma membrane and intracellularly. The causal role of neurone-released glutamate in inducing overexpression of astrocyte CD38 is demonstrated by two observations: first, in the absence of neurones, induction of CD38 in pure astrocyte cultures can be obtained with glutamate and second, it can be prevented in co-cultures by glutamate receptor antagonists. The neuronal glutamate-mediated effect of neurones on astrocyte CD38 expression is paralleled by increased intracellular cADPR and [Ca2+](i) levels, both findings indicating functionality of overexpressed CD38. These results reveal a new neurone-to-astrocyte glutamatergic signalling based on the CD38/cADPR system, which affects the [Ca2+](i) in both cell types, adding further complexity to the bi-directional patterns of communication between astrocytes and neurones. (literal)
Prodotto di
Autore CNR

Incoming links:


Prodotto
Autore CNR di
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it