Paradoxical Ca2+ rises induced by low external Ca2+ in hippocampal neurones. (Articolo in rivista)

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  • Paradoxical Ca2+ rises induced by low external Ca2+ in hippocampal neurones. (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • Burgo A., Carmignoto G., Pizzo P., Pozzan T., Fasolato C. (2003)
    Paradoxical Ca2+ rises induced by low external Ca2+ in hippocampal neurones.
    (literal)
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  • Burgo A., Carmignoto G., Pizzo P., Pozzan T., Fasolato C. (literal)
Pagina inizio
  • 537 (literal)
Pagina fine
  • 552 (literal)
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  • 549 (literal)
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  • pubblicazione su rivista internazionale (literal)
Note
  • ISI Web of Science (WOS) (literal)
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  • Uni PD - Dipartimento di Scienze Biomediche (literal)
Titolo
  • Paradoxical Ca2+ rises induced by low external Ca2+ in hippocampal neurones. (literal)
Abstract
  • Confocal Ca2+ imaging of rat hippocampal slices shows a paradoxical effect of acute reductions of the [Ca2+]o. Upon slice perfusion with low-Ca2+ media, a prompt intracellular Ca2+ rise selectively occurs in neurones. This response is observed only in slices challenged with agonists of group I metabotropic glutamate or M1 muscarinic receptors. In contrast, the intracellular Ca2+ level of non-stimulated neurones is insensitive to reductions of [Ca2+]o. The phenomenon is observed in 20-25 % of cultured cortical neurones. Evidence is provided demonstrating that: (1) this paradoxical response is not due to a non-specific decrease in divalent cation concentration but it is selectively activated by a reduction in [Ca2+]o, being maximal with [Ca2+]o between 0.25 and 0.5 mM; (2) upon maximal stimulation, 70-90 % of CA1-CA3 pyramidal neurones sense a reduction in [Ca2+]o; a weaker response is observed in neurones from the neocortex, whereas neurones from the dentate gyrus and granule cells from the cerebellum fail to respond; (3) conditions that elicit paradoxical Ca2+ responses cause depolarisation and increase the firing rate of hippocampal neurones; (4) paradoxical Ca2+ rises depend, primarily, on Ca2+ influx through L-type voltage-operated Ca2+ channels and to a lesser extent on release from intracellular Ca2+ stores. Inhibition of phospholipase C or protein kinase C failed to suppress the neuronal response, whereas a selective inhibitor of the Src-family of tyrosine kinases abolishes the paradoxical neuronal Ca2+ rise. A model is presented to explain how this response is elicited by contemporaneous reduction of the [Ca2+]o and metabotropic receptor stimulation; implications for the pathophysiology of the CNS are also discussed. (literal)
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