Localization and functional relevance of system a neutral amino acid transporters in cultured hippocampal neurons. (Articolo in rivista)

Type
Label
  • Localization and functional relevance of system a neutral amino acid transporters in cultured hippocampal neurons. (Articolo in rivista) (literal)
Anno
  • 2002-01-01T00:00:00+01:00 (literal)
Alternative label
  • Armano S., Coco S., Bacci A., Pravettoni E., Schenk U, Verderio C., Varoqui H, Erickson J.D., Matteoli M. (2002)
    Localization and functional relevance of system a neutral amino acid transporters in cultured hippocampal neurons.
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Armano S., Coco S., Bacci A., Pravettoni E., Schenk U, Verderio C., Varoqui H, Erickson J.D., Matteoli M. (literal)
Pagina inizio
  • 10467 (literal)
Pagina fine
  • 10473 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 277 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Localization and functional relevance of system a neutral amino acid transporters in cultured hippocampal neurons. (literal)
Abstract
  • Glutamine and alanine are important precursors for the synthesis of glutamate. Provided to neurons by neighboring astrocytes, these amino acids are internalized by classical system A amino acid carriers. In particular, SAT1 is a highly efficient glutamine transporter, whereas SAT2 exhibits broad specificity for neutral amino acids, with a preference for alanine. We investigated the localization and the functional relevance of SAT1 and SAT2 in primary cultures of hippocampal neurons. Both carriers are expressed since early developmental stages and are uniformly distributed throughout all neuronal processes. However, whereas SAT1 is present in axonal growth cones and is detectable, at later developmental stages, at sites of synaptic contacts, SAT2 does not appear to be significantly expressed in these compartments. The nonmetabolizable amino acid analogue _-(methylamino) isobutiric acid, a competitive inhibitor of system A carriers, significantly reduced mEPSC amplitude in neurons growing on top of astrocytes, being uneffective in pure neuronal cultures. _-(methylamino) isobutiric acid did not alter neuronal responsitivity to glutamate, thus excluding a postsynaptic effect. These data indicate that system A carriers are expressed, with a different subcellular distribution, in hippocampal neurons and play a crucial role in controlling the astrocyte-mediated supply of glutamatergic neurons with neurotransmitter precursors. (literal)
Prodotto di

Incoming links:


Prodotto
data.CNR.it