Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study. (Articolo in rivista)

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  • Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study. (Articolo in rivista) (literal)
Anno
  • 2004-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1111/j.1572-0241.2004.30296.x (literal)
Alternative label
  • M.G. Clemente; M.P. Musu; R. Troncone; U. Volta; M.Congia; C. Ciacci; E. Neri; T. Not; G. Maggiore; P. Strisciuglio; G.R. Corazza; G Gasbarrini; L. Cicotto; G. Sole; A. Fasano; S. De Virgiliis. (2004)
    Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study.
    in The American journal of gastroenterology
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • M.G. Clemente; M.P. Musu; R. Troncone; U. Volta; M.Congia; C. Ciacci; E. Neri; T. Not; G. Maggiore; P. Strisciuglio; G.R. Corazza; G Gasbarrini; L. Cicotto; G. Sole; A. Fasano; S. De Virgiliis. (literal)
Pagina inizio
  • 1511 (literal)
Pagina fine
  • 1516 (literal)
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  • 99 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 6 (literal)
Note
  • ISI Web of Science (WOS) (literal)
  • PubMe (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • . Dept. of Biomedical Sciences and Biotechnologies, Second Pediatrics Clinic, Cagliari University, Italy. . Dept. of Pediatrics, University Federico II, Naples, Italy. . Dept. of Internal Medicine, Cardioangiology, Hepatology, S.Orsola-Malpighi Polyclinic, Bologna University, Italy. . Division of Gastroenterology, Federico II University, Naples, Italy. . Dept. of Pediatrics IRCCS Burlo Garofolo, Trieste, Italy. . Dept. of Procreation and Developmental Medicine, University of Pisa, Italy. . Dept. of Paediatrics, University of Catanzaro \"Magna Graecia\", Italy . Gastroenterology Unit, IRCCS Policlinico San Matteo, University of Pavia, Italy. . Dept. of Internal Medicine, Catholic University of the Sacred Heart, Roma, Italy . Mucosal Biology Research Center, University of Maryland School of Medicine, Baltimore, USA. (literal)
Titolo
  • Enterocyte actin autoantibody detection: a new diagnostic tool in celiac disease diagnosis: results of a multicenter study. (literal)
Abstract
  • Objectives. This study describes a new method to detect autoantibodies against actin filaments (AAA) as a serological marker of intestinal villous atrophy (IVA) in celiac disease (CD), and reports the results of an Italian double-blind multicenter study. Methods. IgA-AAA were analysed by immunofluorescence using a newly developed method based on intestinal epithelial cells cultured in presence of colchicine. IgA-AAA were blindly evaluated prospectively in 223 anti-endomysial antibody (AEA) and/or anti- transglutaminase antibody (TGA) positive subjects and in 78 AEA and TGA negative subjects. IgA-AAA positive patients underwent an intestinal biopsy to confirm the diagnosis. Moreover, IgA-AAA were retrospectively investigated in 84 biopsy-proven CD patients and in 2000 new consecutively collected serum samples from AEA and TGA negative non-biopsied subjects. Results. IgA-AAA were positive in 98.2% of the CD patients with flat mucosa, in 89% with subtotal villous atrophy, and in 30% with partial villous atrophy. IgA-AAA were present in none of the AEA and TGA negative non-biopsied controls. In AEA and/or TGA positive CD patients IgA- AAA positivity significantly correlated with IVA (p<0.000 in the prospective study, p=0.005 in the retrospective study). In the prospective study, the values of sensitivity, specificity, the positive predictive value, the negative predictive value and the efficiency of the IgA-AAA test to identify patients with IVA were, respectively, 83.9%, 95.1%, 97.8%, 69.2%, and 87.0%. Furthermore, a significant correlation (p<0.0001) was found between the IgA-AAA serum titre and the degree of IVA (rs 0.56). Conclusions. The results of this multicenter study show that the new method for IgA-AAA detection could represent a practical diagnostic tool in AEA and/or TGA positive subjects, which would be especially useful when IVA shows a patchy distribution, when the histological picture is difficult to interpret, or when a biopsy could represent a life-threatening risk. (literal)
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