Protein conformational changes revealed by optical spectroscopic reflectometry in porous silicon multilayers (Articolo in rivista)

Type
Label
  • Protein conformational changes revealed by optical spectroscopic reflectometry in porous silicon multilayers (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Alternative label
  • De Tommasi E, Rea I, Rendina I, Rotiroti L, De Stefano L (2009)
    Protein conformational changes revealed by optical spectroscopic reflectometry in porous silicon multilayers
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • De Tommasi E, Rea I, Rendina I, Rotiroti L, De Stefano L (literal)
Pagina inizio
  • 035115 (literal)
Pagina fine
  • 035115 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 21 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • IMM-Napoli (literal)
Titolo
  • Protein conformational changes revealed by optical spectroscopic reflectometry in porous silicon multilayers (literal)
Abstract
  • The protein-ligand molecular interactions imply strong geometrical and structural rearrangements of the biological complex which are normally detected by high sensitivity optical techniques such as time-resolved fluorescence microscopy. In this work, we have measured, by optical spectroscopic reflectometry in the visible-near-infrared region, the interaction between a sugar binding protein (SBP), covalently bound on the surface of a porous silicon (PSi) microcavity, and glucose, at different concentrations and temperatures. Variable-angle spectroscopic ellipsometric (VASE) characterization of protein-functionalized PSi layers confirms that the protein-ligand system has an overall volume smaller than the SBP alone. (literal)
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