Experimental characterization of proteins immobilized on Si-based materials (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Experimental characterization of proteins immobilized on Si-based materials (Articolo in rivista) (literal)

Anno

• 2007-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.mee.2006.10.064 (literal)

Alternative label

• Libertino S; Fichera M; Aiello V; Statello G; Fiorenza P; Sinatra F (2007)
  Experimental characterization of proteins immobilized on Si-based materials
  in Microelectronic engineering
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Libertino S; Fichera M; Aiello V; Statello G; Fiorenza P; Sinatra F (literal)

Pagina inizio

• 468 (literal)

Pagina fine

• 473 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 84 (literal)

Rivista

• Microelectronic engineering (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• CNR-IMM Catania, I-95121 Catania, Italy; Catania Univ, Dipartimento Sci Biomed, I-95100 Catania, Italy (literal)

Titolo

• Experimental characterization of proteins immobilized on Si-based materials (literal)

Abstract

• We studied the possibility to use techniques that are traditionally employed in microelectronics to detect biological molecules immobilized on and into Si-based materials having, as final goal, the structural characterization of a glucose biosensor. The inorganic immobilization surfaces used were both bulk and porous silicon dioxide and the biological molecule to monitor was the enzyme glucose oxidase, widely used as a sensing element in glucose biosensors. Bulk SiO2 was used to optimize the immobilization protocol and the step-by-step characterization was mainly carried out by Atomic Force Microscopy measurements. Once optimized, the same protocol was used to anchor the enzyme in a porous Si dioxide matrix. Traditional measurement techniques may fail in biological molecule detection since C, basic element of such biological molecules, is present in Si as a contaminant, or introduced during sample preparation, e.g. for TEM cross section analysis. The enzyme monitoring was carried out by electron diffraction X-ray measurements coupled with scanning electron microscopy and to be sure of the protein presence, it was previously labelled with gold nano particles. We believe that this la (literal)

Prodotto di

• Nuovi sensori in tecnologia planare (MD.P05.008.002) (Modulo)
• Institute for microelectronics and microsystems (IMM) (Istituto)

Autore CNR

• MANUELA FICHERA (Persona)
• PATRICK FIORENZA (Persona)
• SEBANIA LIBERTINO (Unità di personale interno)
• VENERA AIELLO (Unità di personale esterno)

Incoming links:

Autore CNR di

• SEBANIA LIBERTINO (Unità di personale interno)
• VENERA AIELLO (Unità di personale esterno)
• PATRICK FIORENZA (Persona)
• MANUELA FICHERA (Persona)

Prodotto

• Institute for microelectronics and microsystems (IMM) (Istituto)
• Nuovi sensori in tecnologia planare (MD.P05.008.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Microelectronic engineering (Rivista)