http://www.cnr.it/ontology/cnr/individuo/prodotto/ID34120
Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates (Articolo in rivista)
- Type
- Label
- Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1016/j.biomaterials.2007.02.022 (literal)
- Alternative label
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- M. Buttiglione; F. Vitiello; E. Sardella; L. Petrone; M. Nardulli; P. Favia; R. d'Agostino; R. Gristina (literal)
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- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Department of Pharmacology and Human Physiology, University of Bari; Department of Chemistry, University of Bari; Institute of Inorganic Methodologies and Plasmas, IMIP-CNR. (literal)
- Titolo
- Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates (literal)
- Abstract
- Among the parameters that can be tested in experiments on neuronal cell culture the use of different culture media and substrates
represents a powerful assay to influence cell adhesion and differentiation. In this work, plasma-enhanced-chemical vapour depositions
(PE-CVD) from acrylic acid and allylamine vapours have been performed to deposit coatings bearing oxygen (O)- and nitrogen
(N)-containing functional groups on polyethylenetherephtalate (PET) surface. Human neuroblastoma SH-SY5Y cells were grown on
plasma modified substrates and in presence of media containing different amount of fetal calf serum (FCS) or in serum-free medium
containing cAMP. Our results showed that N-containing substrates improved cell adhesion, while the neurites sprouting was influenced
by cell culture media. Interestingly, the presence of carboxylic groups on the modified surface can influence the expression of a
differentiation marker, neurofilament-200 (NF-H), in cells grown in serum-containing media. (literal)
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