Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates (Articolo in rivista)

Type
Label
  • Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates (Articolo in rivista) (literal)
Anno
  • 2007-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1016/j.biomaterials.2007.02.022 (literal)
Alternative label
  • M. Buttiglione; F. Vitiello; E. Sardella; L. Petrone; M. Nardulli; P. Favia; R. d'Agostino; R. Gristina (2007)
    Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates
    in Biomaterials
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • M. Buttiglione; F. Vitiello; E. Sardella; L. Petrone; M. Nardulli; P. Favia; R. d'Agostino; R. Gristina (literal)
Pagina inizio
  • 2932 (literal)
Pagina fine
  • 2945 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 28 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 14 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Pharmacology and Human Physiology, University of Bari; Department of Chemistry, University of Bari; Institute of Inorganic Methodologies and Plasmas, IMIP-CNR. (literal)
Titolo
  • Behaviour of SH-SY5Y neuroblastoma cell line grown in different media and on different chemically modified substrates (literal)
Abstract
  • Among the parameters that can be tested in experiments on neuronal cell culture the use of different culture media and substrates represents a powerful assay to influence cell adhesion and differentiation. In this work, plasma-enhanced-chemical vapour depositions (PE-CVD) from acrylic acid and allylamine vapours have been performed to deposit coatings bearing oxygen (O)- and nitrogen (N)-containing functional groups on polyethylenetherephtalate (PET) surface. Human neuroblastoma SH-SY5Y cells were grown on plasma modified substrates and in presence of media containing different amount of fetal calf serum (FCS) or in serum-free medium containing cAMP. Our results showed that N-containing substrates improved cell adhesion, while the neurites sprouting was influenced by cell culture media. Interestingly, the presence of carboxylic groups on the modified surface can influence the expression of a differentiation marker, neurofilament-200 (NF-H), in cells grown in serum-containing media. (literal)
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