Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode (Articolo in rivista) (literal)

Anno

• 2011-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.aca.2010.11.017 (literal)

Alternative label

• Z. Aturki, G. D’'Orazio, A. Rocco, K. Si-Ahmed, S. Fanali (2011)
  Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode
  in Analytica chimica acta (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Z. Aturki, G. D’'Orazio, A. Rocco, K. Si-Ahmed, S. Fanali (literal)

Pagina inizio

• 103 (literal)

Pagina fine

• 110 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 685 (literal)

Rivista

• Analytica chimica acta (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Metodologie Chimiche, Consiglio Nazionale delle Ricerche, Roma, Italia Laboratoire d'Analyse Organique Fonctionelle, Faculté di Chimie, Alger, Algeria (literal)

Titolo

• Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode (literal)

Abstract

• In this study, the retention and selectivity of a mixture of basic polar drugs were investigated in hydrophilic interaction chromatographic conditions (HILIC) using nano-liquid chromatography(nano-LC). Six sympathomimetic drugs including ephedrine, norephedrine, synephrine, epinephrine,norepinephrine and norphenylephrine were separated by changing experimental parameters such as stationary phase, acetonitrile (ACN) content, buffer pH and concentration, column temperature. Four polar stationary phases (i.e. cyano-, diol-, aminopropyl-silica and Luna HILIC, a cross-linked diol phase) were selected and packed into fused silica capillary columns of 100 micron internal diameter. Among the four stationary phases investigated a complete separation of the all studied compounds was achieved with aminopropyl silica and Luna HILIC stationary phases only. Best chromatographic results were obtained employing a mobile phase composed by ACN/water (92/8, v/v) containing 10mM ammonium formate buffer pH 3. The influence of the capillary temperature on the resolution of the polar basic drugs was investigated in the range between 10 and 50 æC. Enthalpy values were negative with Luna HILIC and positive with aminopropyl- and diol-silica stationary phases, demonstrating that different mechanisms were involved in the separation. (literal)

Prodotto di

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie chimiche e radiochimiche per lo sviluppo di materiali e processi sostenibili (PM.P03.016.001) (Modulo)

Autore CNR

• SALVATORE FANALI (Unità di personale interno)
• ANNA ROCCO (Persona)
• GIOVANNI D'ORAZIO (Unità di personale interno)
• ZEINEB ATURKI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ZEINEB ATURKI (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)
• ANNA ROCCO (Persona)
• GIOVANNI D'ORAZIO (Unità di personale interno)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie chimiche e radiochimiche per lo sviluppo di materiali e processi sostenibili (PM.P03.016.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Analytica chimica acta (Rivista)

Insieme di parole chiave di

• Keywords of "Investigation of polar stationary phases for the separation of sympathomimetic drugs with nano-liquid chromatography in hydrophilic interaction liquid chromatography mode" (Insieme di parole chiave)