Analysis of Aloe-based phytotherapeutic products by using nano-LC-MS (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Analysis of Aloe-based phytotherapeutic products by using nano-LC-MS (Articolo in rivista) (literal)

Anno

• 2010-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/jssc.201000408 (literal)

Alternative label

• S. Fanali, Z. Aturki, G. D'Orazio, A. Rocco, A. Ferranti, L. Mercolini, M. A. Raggi (2010)
  Analysis of Aloe-based phytotherapeutic products by using nano-LC-MS
  in Journal of separation science (Internet)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• S. Fanali, Z. Aturki, G. D'Orazio, A. Rocco, A. Ferranti, L. Mercolini, M. A. Raggi (literal)

Pagina inizio

• 2663 (literal)

Pagina fine

• 2670 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 33 (literal)

Rivista

• Journal of separation science (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto di Metodologie Chimiche (CNR); Istituto di Metodologie Chimiche (CNR); Istituto di Metodologie Chimiche (CNR);Istituto di Metodologie Chimiche (CNR); Department of Pharmaceutical Sciences, Laboratory of Pharmaco-Toxicological Analysis; Department of Pharmaceutical Sciences, Laboratory of Pharmaco-Toxicological Analysis; Department of Pharmaceutical Sciences, Laboratory of Pharmaco-Toxicological Analysis (literal)

Titolo

• Analysis of Aloe-based phytotherapeutic products by using nano-LC-MS (literal)

Abstract

• This article proposes a chromatographic method for the analysis of extracts of Aloe plants. The method was developed with a laboratory assembled nano-LC system coupled with a UV detector, followed by an IT-mass spectrometer. With a step gradient mode of ACN/H2O mixtures and employing a capillary column packed with C18 (100 micron id), a complete separation of the following anthrones was achieved: aloin (in its two isomeric forms A andB), 5-hydroxyaloin and 7-hydroxyaloin (in its two isomeric forms A and B). The optimized nano-LC-MS method was validated for the quantification of aloin, the main component of Aloe with known pharmacological activities. RSD values obtained for retention time and peak areas were 1.3 and 12.1%, respectively. LOD and LOQ values of 0.4 and 1.5 mg/mL were obtained for each aloin isomer. The method was applied to the analysis of Aloe vera and A. ferox extracts in order to acquire a fingerprint, characteristic for each plant. Several phenolic compounds were detected by UV and identified by MS. A. vera and A. ferox showed different profiles and it was possible to discriminate them. Several commercial formulations, declared to contain Aloe extracts, were analyzed. Comparing their chromatograms with those obtained from A. vera and A. ferox, it was possible to recognize the Aloe species and to determine aloin. (literal)

Prodotto di

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie analitiche di caratterizzazione nel settore agro-alimentare (AG.P05.008.001) (Modulo)

Autore CNR

• ZEINEB ATURKI (Unità di personale interno)
• ANNA ROCCO (Persona)
• GIOVANNI D'ORAZIO (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)

Incoming links:

Autore CNR di

• ZEINEB ATURKI (Unità di personale interno)
• SALVATORE FANALI (Unità di personale interno)
• ANNA ROCCO (Persona)
• GIOVANNI D'ORAZIO (Unità di personale interno)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie analitiche di caratterizzazione nel settore agro-alimentare (AG.P05.008.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of separation science (Rivista)