A High Field NMR Study of the products Ensuing from Konjac Glucomannan C(6)-Oxidation followed by Enzymatic C(5)-Epimerization (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• A High Field NMR Study of the products Ensuing from Konjac Glucomannan C(6)-Oxidation followed by Enzymatic C(5)-Epimerization (Articolo in rivista) (literal)

Anno

• 2002-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/bm025613d (literal)

Alternative label

• V. Crescenzi, G. Skjak-Braek, M. Dentini , G. Masci, M.S. Bernalda, D. Risica , D. Capitani, L. Mannina, A.L. Segre (2002)
  A High Field NMR Study of the products Ensuing from Konjac Glucomannan C(6)-Oxidation followed by Enzymatic C(5)-Epimerization
  in Biomacromolecules
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• V. Crescenzi, G. Skjak-Braek, M. Dentini , G. Masci, M.S. Bernalda, D. Risica , D. Capitani, L. Mannina, A.L. Segre (literal)

Pagina inizio

• 1343 (literal)

Pagina fine

• 1352 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://dx.doi.org/10.1021/bm025613d (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 3 (literal)

Rivista

• Biomacromolecules (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 6 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#descrizioneSinteticaDelProdotto

• Studio NMR a campo alto dei prodotti derivanti dal glucomannano Konjac per ossidazione ed epimerizzazione enzimatica.Studio supportato dal progetto Europeo contrtto QLK3-CT-1999-00034 (literal)

Note

• Scopu (literal)
• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Dipartimento di Chimica, Sapienza Università di Roma Istituto di Metodologie Chimiche Dipartimento S.T.A.T:, Università del Molise ,Isernia Department of Biotechnology, Norvegian University of Science and Technology, Trondheim, Norvegia (literal)

Titolo

• A High Field NMR Study of the products Ensuing from Konjac Glucomannan C(6)-Oxidation followed by Enzymatic C(5)-Epimerization (literal)

Abstract

• Konjak glucomannan (KGM) is a water-soluble linear copolymer of (1-4) linked beta-D-mannopyranosyl and beta-D-glucopyranosyl units. It has been selectively C6-oxidized by a 2,2,6,6-tetramethylpiperidin-1-oxy mediated reaction to obtain the corresponding uronan. Oxidized KGM has been treated with three different C-5 epimerases, AlgE4, AlgE6, and AlgE1, to obtain uronans with a various content Of alpha-L-gulopyranuronate residues, namely, KGME4, KGME6, and KGME1 By use of ID selective and 2D NMR techniques, a full assignment of the high field (600 MHz) NMR spectra of the purified native KGM and of the oxidized and epimerized derivatives has been obtained. Since in the anomeric region of the H-1 NMR spectrum of native KGM, diads sensitivity is present, the glucose-glucose, glucose-mannose, mannose-mannose, and mannose-glucose distribution has been obtained. In the C-13 spectrum of oxidized KGM, due to the presence of triad sensitivity on the C-4 resonance of glucuronic and mannuronic units, a better sequential investigation has been possible. As a result the average length of mannuronic blocks, N-M is obtained. When AlgE4, AlgE6, and AlgE1 enzymes are used for the epimerization of oxidized KGM, the reaction products differ significantly both in the proportion and in the distribution of the mannuronic and guluronic residues. In epimerized KGM derivatives, a careful deconvolution of H-1 spectra allows the measurement of the degree of epimerization. In the case of KGME1 and KGME6, the average blocks length, N-G, of the guluronic blocks introduced in the polysaccharidic chain with the epimerization has also been calculated. Due to the shortness of mannuronic blocks in the oxidized KGM before the epimerization, N-G in the epimerized compounds is also very low. (literal)

Prodotto di

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie NMR per lo studio delle scienze omiche (PM.P06.019.003) (Modulo)

Autore CNR

• LUISA MANNINA (Persona)
• ANNA LAURA SEGRE (Unità di personale interno)
• DONATELLA CAPITANI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "A High Field NMR Study of the products Ensuing from Konjac Glucomannan C(6)-Oxidation followed by Enzymatic C(5)-Epimerization" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• DONATELLA CAPITANI (Unità di personale interno)
• LUISA MANNINA (Persona)
• ANNA LAURA SEGRE (Unità di personale interno)

Prodotto

• Istituto di metodologie chimiche (IMC) (Istituto)
• Metodologie NMR per lo studio delle scienze omiche (PM.P06.019.003) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biomacromolecules (Rivista)

Insieme di parole chiave di

• Keywords of "A High Field NMR Study of the products Ensuing from Konjac Glucomannan C(6)-Oxidation followed by Enzymatic C(5)-Epimerization" (Insieme di parole chiave)