Determination of laccase activity in mixed solvents. A comparison between two chromogens in a spectrophotometric assay (Articolo in rivista)

Type
Label
  • Determination of laccase activity in mixed solvents. A comparison between two chromogens in a spectrophotometric assay (Articolo in rivista) (literal)
Anno
  • 2003-01-01T00:00:00+01:00 (literal)
Alternative label
  • Cantarella G., d'Acunzo F., Galli C. (2003)
    Determination of laccase activity in mixed solvents. A comparison between two chromogens in a spectrophotometric assay
    in Biotechnology and bioengineering (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Cantarella G., d'Acunzo F., Galli C. (literal)
Pagina inizio
  • 395 (literal)
Pagina fine
  • 398 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 82 (literal)
Rivista
Note
  • ISI Web of Science (WOS) (literal)
Titolo
  • Determination of laccase activity in mixed solvents. A comparison between two chromogens in a spectrophotometric assay (literal)
Abstract
  • Spectrophotometric determination of laccase activity with ABTS acting as chromogen yields exceedingly low values whenever conducted in a water–organic mixed solvent. Nevertheless, there is firm evidence that laccase is able to oxidize substrates such as phenols and amines quantitatively in these mixed solvents. We show that the apparently small rate of ABTS oxidation by laccase in a mixed solvent, such as buffered water–dioxane 1:1, is not amenable to the denaturation of laccase but rather to the decreased stability of ABTS+. We propose HAA as a more reliable chromogen for the determination of laccase activity in mixed solvents. (literal)
Prodotto di

Incoming links:


Prodotto
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi
data.CNR.it