Osteogenic and osteoclastogenic differentiation of co-cultured cells in polylactic acid-nanohydroxyapatite fiber scaffolds (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Osteogenic and osteoclastogenic differentiation of co-cultured cells in polylactic acid-nanohydroxyapatite fiber scaffolds (Articolo in rivista) (literal)

Anno

• 2015-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.jbiotec.2015.03.023 (literal)

Alternative label

• Sabrina Morelli, Simona Salerno, Jani Holopainen, Mikko Ritala,Loredana De Bartolo (2015)
  Osteogenic and osteoclastogenic differentiation of co-cultured cells in polylactic acid-nanohydroxyapatite fiber scaffolds
  in Journal of biotechnology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Sabrina Morelli, Simona Salerno, Jani Holopainen, Mikko Ritala,Loredana De Bartolo (literal)

Pagina inizio

• 53 (literal)

Pagina fine

• 62 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 204 (literal)

Rivista

• Journal of biotechnology (Rivista)

Note

• PubMe (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute on Membrane Technology, National Research Council of Italy, ITM-CNR, via P. Bucci cubo 17/C, I-87030 Rende, CS Italy Laboratory of Inorganic Chemistry, Department of Chemistry, University of Helsinki, PO Box 55, FI-00014 Helsinki, Finland (literal)

Titolo

• Osteogenic and osteoclastogenic differentiation of co-cultured cells in polylactic acid-nanohydroxyapatite fiber scaffolds (literal)

Abstract

• The design of bone substitutes involves the creation of a microenvironment supporting molecular cross-talk between cells and scaffolds during tissue formation and remodelling. Bone remodelling processincludes the cooperation of bone-building cells and bone-resorbing cells.In this paper we developed polylactic acid (PLA) and composite PLA-nanohydroxyapatite (nHA) scaf-folds with 20 and 50 wt.% of nHA by electrospinning technique to be used in bone tissue engineering.The developed scaffolds have different fiber diameter, porosity with interconnected pores and mechan-ical properties. Taking cues from the bone environment features we investigated the differentiation ofhuman mesenchymal stem cells (hMSCs) from bone marrow in osteoblasts and the osteoclastogenesisin the developed scaffolds in homotypic and in co-culture up to 46 days.PLA and composite PLA-nHA scaffolds induced osteogenic and osteoclastogenic differentiation. Bothosteoblasts and osteoclasts displayed high expression of specific markers (osteopontin, osteocalcin,RANK, RANKL) and functions such as secretion of ALP, cathepsin K and TRAP activity on composite scaf-folds especially on PLA-nHA containing 20 wt.% of nHA. The heterotypic interactions between osteoblastsand osteoclasts co-cultured in the developed scaffolds triggered their functional differentiation andactivation. (literal)

Prodotto di

• Institute on membrane technology (ITM) (Istituto)
• Membrane in organi artificiali (PM.P02.008.001) (Modulo)

Autore CNR

• SABRINA MORELLI (Unità di personale interno)
• LOREDANA DE BARTOLO (Unità di personale interno)
• SIMONA SALERNO (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Osteogenic and osteoclastogenic differentiation of co-cultured cells in polylactic acid-nanohydroxyapatite fiber scaffolds" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute on membrane technology (ITM) (Istituto)
• Membrane in organi artificiali (PM.P02.008.001) (Modulo)

Autore CNR di

• LOREDANA DE BARTOLO (Unità di personale interno)
• SABRINA MORELLI (Unità di personale interno)
• SIMONA SALERNO (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Journal of biotechnology (Rivista)

Insieme di parole chiave di

• Keywords of "Osteogenic and osteoclastogenic differentiation of co-cultured cells in polylactic acid-nanohydroxyapatite fiber scaffolds" (Insieme di parole chiave)