Advances in the definition of a drop-based functionalization protocol for CMOS-compatible MEMS biosensors (Abstract/Poster in convegno)

Type
Label
  • Advances in the definition of a drop-based functionalization protocol for CMOS-compatible MEMS biosensors (Abstract/Poster in convegno) (literal)
Anno
  • 2012-01-01T00:00:00+01:00 (literal)
Alternative label
  • R. Pilolli, N. Ucciferri , V. Russino, N. Ditaranto, L. Tedeschi, N. Cioffi, C. Domenici, A. Nannini, F. Pieri (2012)
    Advances in the definition of a drop-based functionalization protocol for CMOS-compatible MEMS biosensors
    in CONVEGNO NAZIONALE SENSORI - Innovazione, attualità e prospettive, Roma, 15-17 febbraio 2012
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • R. Pilolli, N. Ucciferri , V. Russino, N. Ditaranto, L. Tedeschi, N. Cioffi, C. Domenici, A. Nannini, F. Pieri (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.siof-ottica.it/conv_naz_sens/documenti/programma_web.pdf (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Chimica, Università di Bari, Bari, Italy Istituto di Fisiologia Clinica, Consiglio Nazionale delle Ricerche, Pisa, Italy Dipartimento di Ingegneria dell'Informazione, Università di Pisa, Pisa, Italy (literal)
Titolo
  • Advances in the definition of a drop-based functionalization protocol for CMOS-compatible MEMS biosensors (literal)
Abstract
  • In this work, the use of drop-coating as a substitute to immersion for the creation of bioactive surfaces on MEMS sensors is investigated. The target sensor platform is a CMOS-based resonant sensor based on the microbalance principle. Preliminarily, a test to verify the effectiveness of the functionalization protocol was performed: test silicon dioxide surfaces were cleaned in an ammonia-based hydroxylation solution, and silanized through drop-coating with an aqueous-based APTES (amino-propyl-triethoxysilane) solution as the preliminary step towards the deposition of a bioactive layer. The surfaces were studied by means of conventional and angle resolved x-ray photoelectron spectroscopy (ARXPS). The spectroscopic characterization confirmed that the resulting surface chemical composition was not significantly different upon the two alternative processing approaches: both the atomic percentages values and the outermost layer in-depth distribution of the functionalities are comparable for the two approaches . Then, a sample containing several MEMS resonators underwent a similar procedure. The amino coated resonators were then exposed to a drop of solution containing an oligonucleotide specifically designed to link to a portion of human MGMT (methylguanine-DNA methyltransferase) mRNA, and subsequently to its FITC fluorescent labeled complementary target (again in drop form). A comparison between this sample and a reference sample, not exposed to the target, shows a clear fluorescence signal, and can interpreted as the occurrence of a specific binding between probe and target. Both the XPS and fluorescence data suggest that the drop-based protocol can be successfully used for the bioactivation of MEMS-based biosensors. (literal)
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