Establishment and characterization of a B-cell line derived from a patient with a myelodysplastic syndrome which expresses myelomonocytic and lymphoid markers (Articolo in rivista)

Type
Label
  • Establishment and characterization of a B-cell line derived from a patient with a myelodysplastic syndrome which expresses myelomonocytic and lymphoid markers (Articolo in rivista) (literal)
Anno
  • 1991-01-01T00:00:00+01:00 (literal)
Alternative label
  • Bergamaschi, Gaetano M.; Carlo-Stella, Carmelo Stella; Cattoretti, Giorgio; Invernizzi, Rosangela; Maserati, Emanuela; Nalli, Giulio; Pedrazzoli, Paolo; Peverali, Fiorenzo A A; Della Valle, Giuliano; ASCARI, Edoardo; Cazzola, Mario (1991)
    Establishment and characterization of a B-cell line derived from a patient with a myelodysplastic syndrome which expresses myelomonocytic and lymphoid markers
    in British journal of haematology (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bergamaschi, Gaetano M.; Carlo-Stella, Carmelo Stella; Cattoretti, Giorgio; Invernizzi, Rosangela; Maserati, Emanuela; Nalli, Giulio; Pedrazzoli, Paolo; Peverali, Fiorenzo A A; Della Valle, Giuliano; ASCARI, Edoardo; Cazzola, Mario (literal)
Pagina inizio
  • 167 (literal)
Pagina fine
  • 172 (literal)
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  • http://www.scopus.com/record/display.url?eid=2-s2.0-0025883279&origin=inward (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 78 (literal)
Rivista
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  • 2 (literal)
Note
  • Scopu (literal)
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  • Fondazione IRCCS Policlinico San Matteo (literal)
Titolo
  • Establishment and characterization of a B-cell line derived from a patient with a myelodysplastic syndrome which expresses myelomonocytic and lymphoid markers (literal)
Abstract
  • We describe a novel continuous B-cell line (PV-90) derived from a patient with myelodysplastic syndrome (MDS) and originating from spontaneous infection with the Epstein-Barr virus (EBV). The patient progressed to acute myeloblastic leukaemia (AML) 5 months after clinical onset of MDS. PV-90 is of clonal origin as indicated by the presence of immunoglobulin (Ig) gene rearrangements, monoclonal surface immunoglobulins, and a single DNA restriction fragment corresponding to the EBV genomic termini. PV-90 cells also express a number of myelomonocytic markers, including ?-naphthyl acetate esterase (ANAE), coagulation factor XIII, and CD68 antigen. Moreover, PV-90 cells constitutively express the c-fms proto-oncogene mRNA as the patient's blast cells did. Whereas a trisomy 11 (+11) was found in the patient's bone marrow cells, PV-90 cells had a normal karyotype initially, but at 4 months showed two different and independent chromosomal abnormalities: 90, XX, -Y, -Y, t(9;16) (q11;p13), and 90, XX, -Y, -Y, t(17;18) (p13;q21), the latter possibly involving the p53 (17,p13) and bcl-2 (18, q21) proto-oncogenes. The early development of these chromosomal aberrations is consistent with a genetic instability of PV-90 cells. Expression of bi-lineage markers and genetic instability may suggest that PV-90 cells originated from transformation of a myelodysplastic progenitor cell capable of both myeloid and B-cell differentiation. The PV-90 cell line might be useful in a number of studies, including the possible role of c-fms in cell differentiation, pathogenetic mechanisms of human preleukaemia and lineage promiscuity in acute leukaemia. (literal)
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