Involvement of the Azotobacter vinelandii Rhodanese-like protein RhdA in the glutathione regeneration pathway (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Involvement of the Azotobacter vinelandii Rhodanese-like protein RhdA in the glutathione regeneration pathway (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1371/journal.pone.0045193 (literal)

Alternative label

• William Remelli; Nicoletta Guerrieri; Jennifer Klodmann; Jutta Papenbrock; Silvia Pagani; Fabio Forlani (2012)
  Involvement of the Azotobacter vinelandii Rhodanese-like protein RhdA in the glutathione regeneration pathway
  in PloS one
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• William Remelli; Nicoletta Guerrieri; Jennifer Klodmann; Jutta Papenbrock; Silvia Pagani; Fabio Forlani (literal)

Pagina inizio

• e0045193 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 7 (literal)

Rivista

• PloS one (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 9 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 9 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Milan; University of Hannover; University of Hannover (literal)

Titolo

• Involvement of the Azotobacter vinelandii Rhodanese-like protein RhdA in the glutathione regeneration pathway (literal)

Abstract

• The phenotypic features of the Azotobacter vinelandii RhdA mutant MV474 (in which the rhdA gene was deleted) indicated that defects in antioxidant systems in this organism were related to the expression of the tandem-domain rhodanese RhdA. In this work, further insights on the effects of the oxidative imbalance generated by the absence of RhdA (e.g. increased levels of lipid hydroperoxides) are provided. Starting from the evidence that glutathione was depleted in MV474, and using both in silico and in vitro approaches, here we studied the interaction of wild-type RhdA and Cys(230) Ala site-directed RhdA mutant with glutathione species. We found that RhdA was able to bind in vitro reduced glutathione (GSH) and that RhdA-Cys(230) residue was mandatory for the complex formation. RhdA catalyzed glutathione-disulfide formation in the presence of a system generating the glutathione thiyl radical (GS(.), an oxidized form of GSH), thereby facilitating GSH regeneration. This reaction was negligible when the Cys230 Ala RhdA mutant was used. The efficiency of RhdA as catalyst in GS(.)-scavenging activity is discussed on the basis of the measured parameters of both interaction with glutathione species and kinetic studies. (literal)

Prodotto di

• Istituto per lo studio degli ecosistemi (ISE) (Istituto)
• Impatto dei cambiamenti globali sugli ecosistemi acquatici (TA.P02.014.002) (Modulo)

Autore CNR

• NICOLETTA GUERRIERI (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Involvement of the Azotobacter vinelandii Rhodanese-like protein RhdA in the glutathione regeneration pathway" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per lo studio degli ecosistemi (ISE) (Istituto)
• Impatto dei cambiamenti globali sugli ecosistemi acquatici (TA.P02.014.002) (Modulo)

Autore CNR di

• NICOLETTA GUERRIERI (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• PloS one (Rivista)

Insieme di parole chiave di

• Keywords of "Involvement of the Azotobacter vinelandii Rhodanese-like protein RhdA in the glutathione regeneration pathway" (Insieme di parole chiave)