Time and space evolution of transport properties in agarose - chondrocytes constructs: implication for articular cartilage repair (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Time and space evolution of transport properties in agarose - chondrocytes constructs: implication for articular cartilage repair (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• E. Derosa, F. Urciuolo, C. Borselli, G. Imparato and P.A. Netti (2006)
  Time and space evolution of transport properties in agarose - chondrocytes constructs: implication for articular cartilage repair
  in Tissue engineering
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• E. Derosa, F. Urciuolo, C. Borselli, G. Imparato and P.A. Netti (literal)

Pagina inizio

• 2193 (literal)

Pagina fine

• 2201 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 8 (literal)

Rivista

• Tissue engineering (Rivista)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Institute of Composite and Biomedical Materials (IMCB), National Council Research (CNR), P.Le Tecchio 80, 80125 Naples, Italy Interdisciplinary Research Centre on Biomaterials (CRIB) and Department of Material Engineering and Production (DIMP), University of Naples Federico II, P.le Tecchio 80, 80125 Naples, Italy. (literal)

Titolo

• Time and space evolution of transport properties in agarose - chondrocytes constructs: implication for articular cartilage repair (literal)

Abstract

• During the development of de novo synthesized cartilage tissue engineered constructs, transport and biophysical properties are expected to change in time and space. Monitoring and control of the evolution of these parameters are of crucial importance to process biohybrid constructs in vitro. The aim of this work was to measure fluid and macromolecular transport and evolution of mechanical properties of tissue-engineered cartilage constructs as a function of culture time and extracellular matrix (ECM) production. It was found, in agreement with other literature reports, that mechanical and fluid transport properties of the constructs correlated well with time of culture and glycosaminoglycan (GAG) content. Further, diffusion coefficients of 2 probes, dextran (500 kDa) and bovine serum albumin (BSA), correlated well with GAG production. Diffusion coefficients (D) were measured with high spatial and temporal resolution by fluorescent recovery after photobleaching (FRAP). Diffusivity steadily decreases with time while it does not vary through the thickness of the specimen. On the basis of these results, an empirical relationship between diffusion coefficient and GAG content was proposed for the 2 probes analyzed. The results of this study provide useful information to optimize and control the tissue culture process in vitro. (literal)

Prodotto di

• Institute for composite and biomedical materials (IMCB) (Istituto)
• Biomateriali ed ingegneria dei tessuti (PM.P02.004.002) (Modulo)

Autore CNR

• Francesco Urciuolo (Persona)

Insieme di parole chiave

• Keywords of "Time and space evolution of transport properties in agarose - chondrocytes constructs: implication for articular cartilage repair" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute for composite and biomedical materials (IMCB) (Istituto)
• Biomateriali ed ingegneria dei tessuti (PM.P02.004.002) (Modulo)

Autore CNR di

• Francesco Urciuolo (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Tissue engineering (Rivista)

Insieme di parole chiave di

• Keywords of "Time and space evolution of transport properties in agarose - chondrocytes constructs: implication for articular cartilage repair" (Insieme di parole chiave)