Purification and characterization of a mitochondrial, single-stranded-DNA-binding protein from Paracentrotus lividus eggs (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Purification and characterization of a mitochondrial, single-stranded-DNA-binding protein from Paracentrotus lividus eggs (Articolo in rivista) (literal)

Anno

• 1997-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1111/j.1432-1033.1997.00052.x (literal)

Alternative label

• Roberti, M; Musicco, C; Polosa, PL; Gadaleta, MN; Quagliariello, E; Cantatore, P (1997)
  Purification and characterization of a mitochondrial, single-stranded-DNA-binding protein from Paracentrotus lividus eggs
  in European journal of biochemistry (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Roberti, M; Musicco, C; Polosa, PL; Gadaleta, MN; Quagliariello, E; Cantatore, P (literal)

Pagina inizio

• 52 (literal)

Pagina fine

• 58 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 247 (literal)

Rivista

• European journal of biochemistry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 7 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Bari; Consiglio Nazionale delle Ricerche (CNR) (literal)

Titolo

• Purification and characterization of a mitochondrial, single-stranded-DNA-binding protein from Paracentrotus lividus eggs (literal)

Abstract

• A binding protein for single-stranded DNA was purified from Paracentrotus lividus egg mitochondria to near homogeneity by chromatography on DEAE-Sephacel and single-stranded-DNA-cellulose. The protein consists of a single polypeptide of about 15 kDa. Glycerol gradient sedimentation analysis suggested that P. lividus mitochondrial single-stranded-DNA-binding protein exists as a homo-oligomer, possibly a tetramer, in solution, The protein shows a stronger preference for poly(dT) with respect to single-stranded M13, poly(dI) and poly(dC). Binding to poly(da) takes place with much lower affinity, The binding-site size, determined by gel mobility-shift experiments with oligonucleotides of different length, is approximately 45 nucleotides. The binding to single-stranded DNA occurs with low or no cooperativity and is not influenced by ionic strength. The protein has a very high affinity for the DNA: its apparent macroscopic association constant is 2x10(9) M-1, a value which is the highest among the mitochondrial single-stranded-DNA-binding proteins characterized to date. The lack of cooperativity and the high association constant represent distinctive features of this protein and might be related to the peculiar mechanism of sea urchin mitochondrial DNA replication. (literal)

Prodotto di

• Biogenesi delle Membrane di Trasduzione dell'Energia. (SV.P13.002.001) (Modulo)
• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)

Autore CNR

• CLARA MUSICCO (Persona)
• MARIA NICOLA GADALETA (Persona)
• PALMIRO CANTATORE (Unità di personale esterno)

Insieme di parole chiave

• Parole chiave di "Purification and characterization of a mitochondrial, single-stranded-DNA-binding protein from Paracentrotus lividus eggs" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di biomembrane e bioenergetica (IBBE) (Istituto)
• Biogenesi delle Membrane di Trasduzione dell'Energia. (SV.P13.002.001) (Modulo)

Autore CNR di

• CLARA MUSICCO (Persona)
• MARIA NICOLA GADALETA (Persona)
• PALMIRO CANTATORE (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• European journal of biochemistry (Rivista)

Insieme di parole chiave di

• Parole chiave di "Purification and characterization of a mitochondrial, single-stranded-DNA-binding protein from Paracentrotus lividus eggs" (Insieme di parole chiave)