Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering (Articolo in rivista) (literal)

Anno

• 2006-01-01T00:00:00+01:00 (literal)

Alternative label

• G. Ciapetti, L. Ambrosio, G. Marletta, N. Baldini, A. Giunti (2006)
  Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering
  in Biomaterials
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• G. Ciapetti, L. Ambrosio, G. Marletta, N. Baldini, A. Giunti (literal)

Pagina inizio

• 6150 (literal)

Pagina fine

• 6160 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 27 (literal)

Rivista

• Biomaterials (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note

• 17 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Istituto Ortoprdico Rizzoli, Bologna Università di Catania (literal)

Titolo

• Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering (literal)

Abstract

• Stromal cells from marrow hold a great promise for bone regeneration. Even if they are already being exploited in many clinical settings, the biological basis for the source and maintenance of their proliferation/differentiation potential after in vitro isolation and expansion needs further investigation. Most studies on osteogenic differentiation of marrow stromal cells (MSC) have been performed using bone marrow from the iliac crest. In this study, MSC were derived from spare femoral bone marrow obtained during hip replacement surgery from 20 adult donors. After in vitro isolation the cells were grown in osteogenic medium, and their proliferation and differentiation analysed during in vitro expansion. We found that MSC isolated from the femur of adult patients consistently maintain an osteogenic potential. Using biochemical signals, these cells turn to fully differentiated osteoblasts with a predictable set of molecular and phenotypic events of in vitro bone deposition. When seeded on polycaprolactone-based scaffold or surfaces, the proliferation and mineralization of femurderived MSC were modulated by the surface chemistry/topography. Despite remarkable differences between individual colony-forming ability, alkaline phosphatase production, and mineralization ability, these cells are a potential source for bone engineering, either by direct autologous reimplantation or by ex vivo expansion and reimplantation combined to a proper scaffold. (literal)

Prodotto di

• Biomateriali ed ingegneria dei tessuti (PM.P02.004.002) (Modulo)
• Institute for composite and biomedical materials (IMCB) (Istituto)

Autore CNR

• LUIGI AMBROSIO (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute for composite and biomedical materials (IMCB) (Istituto)
• Biomateriali ed ingegneria dei tessuti (PM.P02.004.002) (Modulo)

Autore CNR di

• LUIGI AMBROSIO (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biomaterials (Rivista)

Insieme di parole chiave di

• Keywords of "Human bone marrow stromal cells: In vitro expansion and differentiation for bone engineering" (Insieme di parole chiave)