Role of the liquid phase on the separation of biomolecules by HPLC and capillary electrophoresis: fundamental and practical aspects (Abstract/Comunicazione in atti di convegno)

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  • Role of the liquid phase on the separation of biomolecules by HPLC and capillary electrophoresis: fundamental and practical aspects (Abstract/Comunicazione in atti di convegno) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Alternative label
  • Danilo Corradini, Isabella Nicoletti (2013)
    Role of the liquid phase on the separation of biomolecules by HPLC and capillary electrophoresis: fundamental and practical aspects
    in 19th International Symposium on Separation Sciences - New Achievements in Chromatography, Porec, Croatia, 25-28 Settembre 2013
    (literal)
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  • Danilo Corradini, Isabella Nicoletti (literal)
Pagina inizio
  • 20 (literal)
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  • Il contributo è stato presentato dal dott. Danilo Corradini (literal)
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  • Book of Abstracts 19th International Symposium on Separation Sciences - New Achievements in Chromatography (literal)
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  • 1 (literal)
Note
  • Comunicazione (literal)
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  • CNR - Istituto di Metodologie Chimiche (literal)
Titolo
  • Role of the liquid phase on the separation of biomolecules by HPLC and capillary electrophoresis: fundamental and practical aspects (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#isbn
  • 978-953-6470-64-8 (literal)
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  • ?ime Uki? and Tomislav Bolan?a (literal)
Abstract
  • Most chromatographic and electrophoretic methods used for the analytical separationof biomolecules are performed in aqueous solutions whose composition might affect to different extent selectivity, resolution and efficiency. This aspect is particularly relevant for a significant number of biomolecules bearing different concomitant functionalities, consisting of ionisable and/or hydrogen-bonding groups, hydrophobic regions, and hydrophilic moieties. Such multifunctional molecules may interact to different extents with the various components of the surrounding aqueous solution and with either the stationary phase or the capillary wall, in chromatography and in capillary electrophoresis, respectively. This communication discusses the results of our recent studies conducted to investigate a variety of factors that influence both electrophoretic and chromatographic behaviour of biomolecules of particular interest in plant physiology and food chemistry. The presentation evaluates the influence of the composition of either the electrolyte solution (BGE) or the mobile phase on the selective separation of representative plant secondary metabolites in capillary zone electrophoresis (CZE) and in reversed phase liquid chromatography (RP-HPLC), respectively. Appropriate selection of the composition of either the BGE or the mobile phase involves the evaluation of the equilibrium in solution that might take place between the analytes and the components of such solutions. The result is the possibility of tailoring selectivity and efficiency of the considered separation systems by incorporating suitable buffering agents and additives into the BGE or the mobile phase, respectively. Practical applications of these approaches to the analysis of phenolic compounds in samples extracted from a variety of plant tissues and food of vegetable origin are then discussed. (literal)
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