Identification and quantification of phenolic acids in durum wheat by RP-HPLC on a semimicro separation scale with PDA and ESI-MS detection (Abstract/Poster in convegno)

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Label
  • Identification and quantification of phenolic acids in durum wheat by RP-HPLC on a semimicro separation scale with PDA and ESI-MS detection (Abstract/Poster in convegno) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • I. Nicoletti, D. Martini, F. Taddei, R. Ciccoritti, M. G. D'Egidio, D. Corradini (2014)
    Identification and quantification of phenolic acids in durum wheat by RP-HPLC on a semimicro separation scale with PDA and ESI-MS detection
    in 38th International Symposium on Capillary Chromatography and 11th GCx GC Symposium, Riva del Garda (TN), 18-23 Maggio 2014
    (literal)
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  • I. Nicoletti, D. Martini, F. Taddei, R. Ciccoritti, M. G. D'Egidio, D. Corradini (literal)
Pagina inizio
  • 492 (literal)
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  • Abstract Book of 38th International Symposium on Capillary Chromatography and 11th GCx GC Symposium (literal)
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  • 1 (literal)
Note
  • Poster (literal)
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  • Consiglio per la Ricerca e la Sperimentazione in Agricoltura (CRA) -Unità di Ricerca per la Valorizzazione Qualitativa dei Cereali), Università Campus Bio-Medico, CNR - Istituto di Metodologie Chimiche (literal)
Titolo
  • Identification and quantification of phenolic acids in durum wheat by RP-HPLC on a semimicro separation scale with PDA and ESI-MS detection (literal)
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  • L. Mondello (literal)
Abstract
  • Durum wheat is one of the most important cereal crops used in the Mediterranean-type temperate zones whose consumption might reduce the risk of developing chronic diseases, such as cardiovascular diseases, type-2 diabetes, and certain types of cancer. These health benefits have been partly attributed to the occurrence of phytochemicals with antioxidant activity, which include phenolic acids (PAs) belonging to hydroxycinnamic acids and hydroxybenzoic acids derivatives. They occur as soluble free acids, soluble conjugates PAs that are esterified to sugars and other low molecular mass compounds, and as insoluble bound PAs that are mostly ester-linked to cell wall polymers such as polysaccharides and lignin. This communication reports the results of a study performed to identifying and quantifying PAs occurring as soluble free, soluble conjugated and insoluble bound compounds, which were separately extracted from wholemeal of durum wheat of different genotypes cultivated in three different Italian regions over three consecutive crop years. The three forms of PAs were independently identified and quantified by HPLC, using a narrow bore reversed phase column and a semimicro photodiode array detector (PDA) cell, in conjunction with a single quadrupole mass spectrometer, equipped with an electrospray ionization source (ESI-MS). The method was validated in terms of linearity of calibration graphs, limits of detection, limits of quantification, repeatability and accuracy, which was evaluated by a recovery study. Our study has evidenced that RP-HPLC on a semimicro separation scale offers both economical and environmental benefits, while maintaining separation performance and reliability of traditional HPLC methods, allowing the accurate estimation of the individual PAs occurring as soluble free, soluble conjugated and insoluble bound compounds in wholemeal of durum wheat. The variations in the content of the three forms of PAs in durum wheat as a function of genotype, cultivation region and crop year are discussed. (literal)
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