Identification and quantification of phenolic compounds in leaves of Cyclanthera Pedata (L.) (Caigua) by reversedv phase HPLC on a semimicro separation scale with photodiode array and mass spectrometric detection (Abstract/Poster in convegno)

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  • Identification and quantification of phenolic compounds in leaves of Cyclanthera Pedata (L.) (Caigua) by reversedv phase HPLC on a semimicro separation scale with photodiode array and mass spectrometric detection (Abstract/Poster in convegno) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • L. Aruanno, I. Nicoletti, L. De Gara, D. Corradini (2014)
    Identification and quantification of phenolic compounds in leaves of Cyclanthera Pedata (L.) (Caigua) by reversedv phase HPLC on a semimicro separation scale with photodiode array and mass spectrometric detection
    in 38th International Symposium on Capillary Chromatography and 11th GCx GC Symposium, Riva del Garda (TN), 18-23 Maggio 2014
    (literal)
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  • L. Aruanno, I. Nicoletti, L. De Gara, D. Corradini (literal)
Pagina inizio
  • 266 (literal)
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  • Abstract Book of 38th International Symposium on Capillary Chromatography and 11th GCx GC Symposium (literal)
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  • 1 (literal)
Note
  • Poster (literal)
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  • Università Campus Biomedico, Roma, Italy; CNR- Istituto di Metodologie Chimiche (literal)
Titolo
  • Identification and quantification of phenolic compounds in leaves of Cyclanthera Pedata (L.) (Caigua) by reversedv phase HPLC on a semimicro separation scale with photodiode array and mass spectrometric detection (literal)
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  • L. Mondello (literal)
Abstract
  • Phenolic compounds are secondary metabolites, which are widely distributed in the plant kingdom and therefore form an integral part of human diet. A growing interest has been addressed to this class of compounds because clinical and epidemiological studies have suggested that the regular consumption of fruits and edible plants might reduce the risk of developing chronic illnesses such as cardiovascular diseases, type-2 diabetes, and certain types of cancer. This communication reports the results of a study carried out to develop a straightforward HPLC method for the identification and quantification of phenolic compounds occurring in the leaves of Cyclanthera pedata Scrabs (Caigua). This is an ancient Peruvian edible plant belonging to the Cucurbitaceae family, which have an increasing commercial interest in the phytopharmaceutical market due to its anti-inflammatory, hypoglycaemic and hypocholesterolemic properties. The method employs a narrow-bore C-18 column and a semimicro photodiode array detector (PDA) cell of 2.5 ?L, in conjunction with a single quadrupole mass spectrometer, equipped with an electrospray ionization source (ESI-MS). The C-18 narrow bore column is eluted by a multi-segment gradient of increasing concentration of acetonitrile in water-formic acid solution that has been optimized on the basis of the results of a study carried out to evaluate the influence of mobile phase composition and gradient shape on separation performance and detection sensitivity by ESI-MS. The identification of individual phenolic compounds has been performed on the basis of their retention times and both UVvisible and mass spectra, acquired by PDA and ESI-MS, respectively. Different organic solvents, hydro-organic mixtures, and plain water have been tested for their efficiency for extraction of phenolic compounds from leaves of Cyclanthera pedata either grown in a green house, under controlled conditions of humidity, temperature and exposure to light, or in an experimental field in Rome geographical area. (literal)
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