Molecular characterization and functional analysis of four beta-1,4-endoglucanases from the root-lesion nematode Pratylenchus vulnus (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Molecular characterization and functional analysis of four beta-1,4-endoglucanases from the root-lesion nematode Pratylenchus vulnus (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1111/ppa.12222 (literal)

Alternative label

• Fanelli E., Troccoli A., Picardi E., Pousis C., De Luca F. (2014)
  Molecular characterization and functional analysis of four beta-1,4-endoglucanases from the root-lesion nematode Pratylenchus vulnus
  in Plant pathology (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Fanelli E., Troccoli A., Picardi E., Pousis C., De Luca F. (literal)

Pagina inizio

• 1436 (literal)

Pagina fine

• 1445 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 63 (literal)

Rivista

• Plant pathology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 6 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Picardi E.: Dipartimento di Bioscienze, Biotecnologie e Scienze Farmacologiche, Università degli Studi di Bari, 70126 Bari, Italy (literal)

Titolo

• Molecular characterization and functional analysis of four beta-1,4-endoglucanases from the root-lesion nematode Pratylenchus vulnus (literal)

Abstract

• This study reports on the isolation and characterization of four different endoglucanases in the root-lesion nematode Pratylenchus vulnus. The gene structures of two of these, Pv-eng-1 and Pv-eng-2, were fully determined, and Pv-eng-3 and Pv-eng-5 were partially sequenced. Spatial expression of Pv-eng-1, Pv-eng-2 and Pv-eng-5 was examined by in situ hybridization. Pv-eng-1 and Pv-eng-2 transcripts were localized in the subventral oesophageal glands, whilst the Pv-eng-5 transcript was localized in the intestine. Real-time RT-PCR showed that three of the four endoglucanases had the highest transcriptional level in adult males and females, thus demonstrating that adults are also parasitic in P.vulnus. Mixed stages of P.vulnus were treated with double-stranded RNA (dsRNA) of Pv-eng-1 in order to study the effect of gene silencing (RNAi). Silencing Pv-eng-1 by dsRNA targeting of the carbohydrate-binding module (CBM) resulted in a significant reduction (88-98%) of the transcript level, suggesting that P.vulnus is susceptible to RNAi. Furthermore, silencing P.vulnus showed a reduction (54%) in nematode reproduction on carrot minidiscs over a 5week period. These results suggest that silencing of Pv-eng-1 may result in reduction of the ability of the nematode to locate and invade roots and, therefore, to establish and reproduce. (literal)

Prodotto di

• Istituto per la protezione delle piante (IPP) (Istituto)
• Tecnologie ecosostenibili per la protezione delle piante dagli agenti di danno (AG.P04.026.001) (Modulo)

Autore CNR

• Chrysovalentinos POUSIS (Unità di personale esterno)
• ELENA FANELLI (Unità di personale esterno)
• FRANCESCA DE LUCA (Persona)
• ALBERTO TROCCOLI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Molecular characterization and functional analysis of four beta-1,4-endoglucanases from the root-lesion nematode Pratylenchus vulnus" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto per la protezione delle piante (IPP) (Istituto)
• Tecnologie ecosostenibili per la protezione delle piante dagli agenti di danno (AG.P04.026.001) (Modulo)

Autore CNR di

• ALBERTO TROCCOLI (Unità di personale interno)
• FRANCESCA DE LUCA (Persona)
• ELENA FANELLI (Unità di personale esterno)
• Chrysovalentinos POUSIS (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Plant pathology (Rivista)

Insieme di parole chiave di

• Parole chiave di "Molecular characterization and functional analysis of four beta-1,4-endoglucanases from the root-lesion nematode Pratylenchus vulnus" (Insieme di parole chiave)