Mitochondrial Ca2+ uptake contributes to buffering cytoplasmic Ca2+ peaks in cardiomyocytes (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Mitochondrial Ca2+ uptake contributes to buffering cytoplasmic Ca2+ peaks in cardiomyocytes (Articolo in rivista) (literal)

Anno

• 2012-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1073/pnas.1210718109 (literal)

Alternative label

• Drago I.; De Stefani D.; Rizzuto R.; Pozzan T. (2012)
  Mitochondrial Ca2+ uptake contributes to buffering cytoplasmic Ca2+ peaks in cardiomyocytes
  in Proceedings of the National Academy of Sciences of the United States of America
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Drago I.; De Stefani D.; Rizzuto R.; Pozzan T. (literal)

Pagina inizio

• 12986 (literal)

Pagina fine

• 12991 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.ncbi.nlm.nih.gov/pubmed/22822213 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 109 (literal)

Rivista

• Proceedings of the National Academy of Sciences of the United States of America (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 32 (literal)

Note

• ISI Web of Science (WOS) (literal)
• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1, 2: Department of Biomedical Sciences, University of Padua, 35121, Italy; 3: Department of Biomedical Sciences, University of Padua, 35121, Italy / Institute of Neurosciences, Italian National Research Council, Padua Section, 35121 Padova, Italy; 4: Department of Biomedical Sciences, University of Padua, 35121, Italy / Institute of Neurosciences, Italian National Research Council, Padua Section, 35121 Padua, Italy / Venetian Institute of Molecular Medicine, 35121 Padua, Italy (literal)

Titolo

• Mitochondrial Ca2+ uptake contributes to buffering cytoplasmic Ca2+ peaks in cardiomyocytes (literal)

Abstract

• Mitochondrial ability of shaping Ca2+ signals has been demonstrated in a large number of cell types, but it is still debated in heart cells. Here, we take advantage of the molecular identification of the mitochondrial Ca2+ uniporter (MCU) and of unique targeted Ca2+ probes to directly address this issue. We demonstrate that, during spontaneous Ca2+ pacing, Ca2+ peaks on the outer mitochondrial membrane (OMM) are much greater than in the cytoplasm because of a large number of Ca2+ hot spots generated on the OMM surface. Cytoplasmic Ca2+ peaks are reduced or enhanced by MCU overexpression and siRNA silencing, respectively; the opposite occurs within the mitochondrial matrix. Accordingly, the extent of contraction is reduced by overexpression of MCU and augmented by its down-regulation. Modulation of MCU levels does not affect the ATP content of the cardiomyocytes. Thus, in neonatal cardiac myocytes, mitochondria significantly contribute to buffering the amplitude of systolic Ca2+ rises. (literal)

Prodotto di

• Neurobiologia e Neuropatologia (ME.P02.005.001) (Modulo)
• Istituto di neuroscienze (IN) (Istituto)

Autore CNR

• TULLIO POZZAN (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Mitochondrial Ca2+ uptake contributes to buffering cytoplasmic Ca2+ peaks in cardiomyocytes" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di neuroscienze (IN) (Istituto)
• Neurobiologia e Neuropatologia (ME.P02.005.001) (Modulo)

Autore CNR di

• TULLIO POZZAN (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Proceedings of the National Academy of Sciences of the United States of America (Rivista)

Insieme di parole chiave di

• Keywords of "Mitochondrial Ca2+ uptake contributes to buffering cytoplasmic Ca2+ peaks in cardiomyocytes" (Insieme di parole chiave)