Thyroid Cancer Imaging In Vivo by Targeting the Anti-Apoptotic Molecule Galectin-3 (Articolo in rivista)

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  • Thyroid Cancer Imaging In Vivo by Targeting the Anti-Apoptotic Molecule Galectin-3 (Articolo in rivista) (literal)
Anno
  • 2008-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1371/journal.pone.0003768 (literal)
Alternative label
  • Bartolazzi, A.; D'Alessandria C.; Parisella, M.; Signore A.; Del Prete F.; Lavra, L.; Braesch-Andersen S.; Massari R.; Trotta, C.; Soluri A.; Sciacchitano S.; and Scopinaro, F. (2008)
    Thyroid Cancer Imaging In Vivo by Targeting the Anti-Apoptotic Molecule Galectin-3
    in PloS one
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Bartolazzi, A.; D'Alessandria C.; Parisella, M.; Signore A.; Del Prete F.; Lavra, L.; Braesch-Andersen S.; Massari R.; Trotta, C.; Soluri A.; Sciacchitano S.; and Scopinaro, F. (literal)
Pagina inizio
  • e3768 (literal)
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  • http://www.plosone.org (literal)
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  • 3 (literal)
Rivista
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  • 6 (literal)
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  • 11 (literal)
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  • Scopu (literal)
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Bartolazzi, Del Prete: Cellular and Molecular Tumor Pathology Laboratory, Cancer Center Karolinska, Karolinska Hospital, Stockholm, Sweden and Pathology Research Laboratory, St. Andrea University Hospital, Rome, Italy / D'Alessandria, Parisella, Signore, Scopinaro: Laboratory of Nuclear Medicine, St. Andrea University Hospital, University La Sapienza, Rome, Italy / Lavra, Sciacchitano: S. Pietro Fatebenefratelli Hospital -Associazione Fatebenefratelli per la Ricerca (AfaR), Rome, Italy and Department of Experimental Medicine and Pathology, II Faculty of Medicine, University ''La Sapienza'', Rome, Italy / Braesch-Andersen: Mabtech Research Laboratory, Nacka, Sweden / Massari, Trotta, Soluri: Istituto di Ingegneria Biomedica (ISIB) - National Council of Research, Sezione di Roma, Italy / (literal)
Titolo
  • Thyroid Cancer Imaging In Vivo by Targeting the Anti-Apoptotic Molecule Galectin-3 (literal)
Abstract
  • Abstract Background: The prevalence of thyroid nodules increases with age, average 4-7% for the U.S.A. adult population, but it is much higher (19-67%) when sub-clinical nodules are considered. About 90% of these lesions are benign and a reliable approach to their preoperative characterization is necessary. Unfortunately conventional thyroid scintigraphy does not allow the distinction among benign and malignant thyroid proliferations but it provides only functional information (cold or hot nodules). The expression of the anti-apoptotic molecule galectin-3 is restricted to cancer cells and this feature has potential diagnostic and therapeutic implications. We show here the possibility to obtain thyroid cancer imaging in vivo by targeting galectin-3. Methods: The galectin-3 based thyroid immuno-scintigraphy uses as radiotracer a specific 99mTc-radiolabeled mAb. A position-sensitive high-resolution mini-gamma camera was used as imaging capture device. Human galectin-3 positive thyroid cancer xenografts (ARO) and galectin-3 knockout tumors were used as targets in different experiments in vivo. 38 mice with tumor mass of about 1 gm were injected in the tail vein with 100 mCi of 99mTc-labeled mAb to galectin-3 (30 mg protein/in 100 ml saline solution). Tumor images were acquired at 1 hr, 3 hrs, 6 hrs, 9 hrs and 24 hrs post injection by using the mini-gamma camera. Findings: Results from different consecutive experiments show an optimal visualization of thyroid cancer xenografts between 6 and 9 hours from injection of the radiotracer. Galectin-3 negative tumors were not detected at all. At 6 hrs postinjection galectin-3 expressing tumors were correctly visualized, while the whole-body activity had essentially cleared. Conclusions: These results demonstrate the possibility to distinguish preoperatively benign from malignant thyroid nodules by using a specific galectin-3 radio-immunotargeting. In vivo imaging of thyroid cancer may allow a better selection of patients referred to surgery. The possibility to apply this method for imaging and treatment of other galectin-3 expressing tumors is also discussed. (literal)
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