http://www.cnr.it/ontology/cnr/individuo/prodotto/ID301225

Impairment of acquisition of intravenous cocaine self-administration by RNA-interference of dopamine D1-receptors in the nucleus accumbens shell (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Impairment of acquisition of intravenous cocaine self-administration by RNA-interference of dopamine D1-receptors in the nucleus accumbens shell (Articolo in rivista) (literal)

Anno

2015-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1016/j.neuropharm.2014.10.018 (literal)

Alternative label

Pisanu A.; Lecca D.; Valentini V.; Bahi A.; Dreyer J.-L.; Cacciapaglia F.; Scifo A.; Piras G.; Cadoni C.; Di Chiara G. (2015)
Impairment of acquisition of intravenous cocaine self-administration by RNA-interference of dopamine D1-receptors in the nucleus accumbens shell
in Neuropharmacology (Online)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Pisanu A.; Lecca D.; Valentini V.; Bahi A.; Dreyer J.-L.; Cacciapaglia F.; Scifo A.; Piras G.; Cadoni C.; Di Chiara G. (literal)

Pagina inizio

398 (literal)

Pagina fine

411 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.scopus.com/inward/record.url?eid=2-s2.0-84918589195&partnerID=q2rCbXpz (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

89 (literal)

Rivista

Neuropharmacology (Rivista)

Note

Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Institute of Neuroscience, National Research Council of Italy, Cagliari, 09124, Italy; Department of Biomedical Sciences, Neuropsychopharmacology Section, University of Cagliari, Via Ospedale 72, Cagliari, 09124, Italy; National Institute of Neuroscience, University of Cagliari, Cagliari, 09124, Italy; Centre of Excellence 'Neurobiology of Dependence', University of Cagliari, Cagliari, 09124, Italy; Division of Biochemistry, Department of Medicine, University of Fribourg, Fribourg, CH-1700, Switzerland; Department of Toxicology, University of Cagliari, Cagliari, 09124, Italy; Department of Psychology, College of Arts AndSciences, University of Nebraska, Lincoln, NE, United States (literal)

Titolo

Impairment of acquisition of intravenous cocaine self-administration by RNA-interference of dopamine D1-receptors in the nucleus accumbens shell (literal)

Abstract

Microdialysis during i.v. drug self-administration (SA) have implicated nucleus accumbens (NAc) shell DA in cocaine and heroin reinforcement. However, this correlative evidence has not been yet substantiated by experimental evidence obtained by studying the effect of selective manipulation of NAc shell DA transmission on cocaine and heroin SA. In order to investigate this issue, DA D1a receptor (D1aR) expression was impaired in the NAc shell and core by locally infusing lentiviral vectors (LV) expressing specific D1aR-siRNAs (LV-siRNAs). Control rats were infused in the same areas with LV expressing GFP. Fifteen days later, rats were trained to acquire i.v. cocaine or heroin self-administration (SA). At the end of behavioral experiments, in order to evaluate the effect of LV-siRNA on D1aR expression, rats were challenged with amphetamine and the brains were processed for immunohistochemical detection of c-Fos and D1aR. Control rats acquired i.v. cocaine and heroin SA. Infusion of LV-siRNAs in the medial NAc shell reduced D1aR density and the number of c-Fos positive nuclei in the NAc shell, while sparing the core, and prevented the acquisition of cocaine, but not heroin SA. In turn, LV-siRNAs infusion in the core reduced D1aR density and the number of c-Fos positive nuclei in the same area, while sparing the shell, and failed to affect acquisition of cocaine. The differential effect of LV impairment of NAc shell D1aR on cocaine and heroin SA indicates that NAc shell DA acting on D1aR specifically mediates cocaine reinforcement. (literal)

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