Cations as Switches of Amyloid-Mediated Membrane Disruption Mechanisms: Calcium and IAPP (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Cations as Switches of Amyloid-Mediated Membrane Disruption Mechanisms: Calcium and IAPP (Articolo in rivista) (literal)

Anno

• 2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.bpj.2012.11.3811 (literal)

Alternative label

• Sciacca, Michele F. M.; Milardi, Danilo; Messina, Garcia M. L.; Marletta, Giovanni; Brender, Jeffrey R.; Ramamoorthy, Ayyalusamy; La Rosa, Carmelo (2013)
  Cations as Switches of Amyloid-Mediated Membrane Disruption Mechanisms: Calcium and IAPP
  in Biophysical journal (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Sciacca, Michele F. M.; Milardi, Danilo; Messina, Garcia M. L.; Marletta, Giovanni; Brender, Jeffrey R.; Ramamoorthy, Ayyalusamy; La Rosa, Carmelo (literal)

Pagina inizio

• 173 (literal)

Pagina fine

• 184 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 104 (literal)

Rivista

• Biophysical journal (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 12 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Michigan; University of Catania; University of Catania (literal)

Titolo

• Cations as Switches of Amyloid-Mediated Membrane Disruption Mechanisms: Calcium and IAPP (literal)

Abstract

• Disruption of the integrity of the plasma membrane by amyloidogenic proteins is linked to the pathogenesis of a number of common age-related diseases. Although accumulating evidence suggests that adverse environmental stressors such as unbalanced levels of metal ions may trigger amyloid-mediated membrane damage, many features of the molecular mechanisms underlying these events are unknown. Using human islet amyloid polypeptide (hIAPP, aka amylin), an amyloidogenic peptide associated with beta-cell death in type 2 diabetes, we demonstrate that the presence of Ca2+ ions inhibits membrane damage occurring immediately after the interaction of freshly dissolved hIAPP with the membrane, but significantly enhances fiber-dependent membrane disruption. In particular, dye leakage, quartz crystal microbalance, atomic force microscopy, and NMR experiments show that Ca2+ ions promote a shallow membrane insertion of hIAPP, which leads to the removal of lipids from the bilayer through a detergent-like mechanism triggered by fiber growth. Because both types of membrane-damage mechanisms are common to amyloid toxicity by most amyloidogenic proteins, it is likely that unregulated ion homeostasis, amyloid aggregation, and membrane disruption are all parts of a self-perpetuating cycle that fuels amyloid cytotoxicity. (literal)

Prodotto di

• Progettazione e sintesi di nuovi composti bioattivi e loro veicolazione sito specifica. (PM.P01.003.002) (Modulo)
• Institute of biostructure and bioimaging (IBB) (Istituto)

Autore CNR

• DANILO MILARDI (Persona)

Insieme di parole chiave

• Keywords of "Cations as Switches of Amyloid-Mediated Membrane Disruption Mechanisms: Calcium and IAPP" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• DANILO MILARDI (Persona)

Prodotto

• Institute of biostructure and bioimaging (IBB) (Istituto)
• Progettazione e sintesi di nuovi composti bioattivi e loro veicolazione sito specifica. (PM.P01.003.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Biophysical journal (Rivista)

Insieme di parole chiave di

• Keywords of "Cations as Switches of Amyloid-Mediated Membrane Disruption Mechanisms: Calcium and IAPP" (Insieme di parole chiave)