Molecular and phenotypic characterization of human amniotic fluid-derived cells. A morphological and proteomic approach. (Abstract/Poster in atti di convegno)

Type
Label
  • Molecular and phenotypic characterization of human amniotic fluid-derived cells. A morphological and proteomic approach. (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • C. Pipino,1,2 L. Pierdomenico,1,2 P. Di Tomo,1,2 F. Di Giuseppe,1,2 E. Cianci,1,2 I. D'Alimonte,1,2 C. Morabito,1,2 L. Centurione,1,2 I. Antonucci,1,2 M.A. Centurione,1,2,3 M.A. Mariggiò,1,2 R. Ciccarelli,1,2 M. Marchisio,1,2 M. Romano,1,2 S. Angelucci,1,2 A. Pandolfi.1,2 (2014)
    Molecular and phenotypic characterization of human amniotic fluid-derived cells. A morphological and proteomic approach.
    in Stem Cell Research Italy International Society for Cellular Therapy-Europe AICC JOINT MEETING, salerno, 28-29-30 maggio 2014
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • C. Pipino,1,2 L. Pierdomenico,1,2 P. Di Tomo,1,2 F. Di Giuseppe,1,2 E. Cianci,1,2 I. D'Alimonte,1,2 C. Morabito,1,2 L. Centurione,1,2 I. Antonucci,1,2 M.A. Centurione,1,2,3 M.A. Mariggiò,1,2 R. Ciccarelli,1,2 M. Marchisio,1,2 M. Romano,1,2 S. Angelucci,1,2 A. Pandolfi.1,2 (literal)
Note
  • Poster (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1University G. d'Annunzio Chieti-Pescara; 2StemTeCh Group, Chieti; 3Institute of Molecular Genetics, National Research Council, Chieti, Italy. (literal)
Titolo
  • Molecular and phenotypic characterization of human amniotic fluid-derived cells. A morphological and proteomic approach. (literal)
Abstract
  • Mesenchymal Stem Cells derived from Amniotic Fluid (AFMSCs) are multipotent cells able to give rise to multiple mesenchymal lineages and therefore of great interest for regenerative medicine. Although the presence of different cell populations in the amniotic fluid has been documented, the two predominant cell types, i.e. Epithelial-like (E-like) and Fibroblast-like (F-like), need to be fully characterized. In the present study, we isolated the cell populations from second trimester amniotic fluid of pregnant women and classified as E-like or F-like phenotype (by phase contrast and toluidine blue-stained semithin section microscopy) on the basis of the prevalence (60-70%) of epithelial or fibroblast morphology of the cultured cells, respectively. Importantly, we tested the immunophenotype of cultured AFMSCs by flow cytometry analysis using a large types of markers. These phenotypes showed slight differences in membrane markers, with higher CD90 and lower Sox2 and SSEA-4 expression in F-like compared to E-like cells, whereas CD326 was expressed only in the E-like phenotype. Nevertheless, they displayed similar capability to differentiate into osteogenic and adipogenic lineages. In fact both E-like and F-like AFMSCs maintained in osteogenic medium, exhibited a greater time-dependent (7-14-21 days) increase in calcium deposition compared to cells kept in normal medium and a comparable increment in Oil Red O staining as well as in the presence of single adipocytes with multiple vacuoles. Furthermore, proteomic analysis revealed proteins specifically expressed in each cluster (HC1, epithelial; HC2, fibroblast). Of note, twenty-five and eighteen protein spots were differentially expressed in HC1 and HC2 classes, respectively. The protein-interaction networks (PIN) for both phenotypes, showed strong interactions between specific AFMSC proteins and molecular chaperones, such as pre-proteosomes and mature proteosomes, both important for cell cycle regulation and apoptosis. Thus, our results provide the first evidence that, although cultured E-like and F-like AFMSCs displayed a differential proteomic phenotype, they showed similar in vitro properties. Then, proteomic profile of E-like and F-like AFMSCs could be uncoupled from the differentiation potential and therefore both cell phenotype might be considered an excellent source for basic research and for potential therapeutic use. (literal)
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