http://www.cnr.it/ontology/cnr/individuo/prodotto/ID293669

Grapevine leafroll-associated virus 3 (Articolo in rivista)

Type

Prodotto della ricerca (Classe)
Articolo in rivista (Classe)

Label

Grapevine leafroll-associated virus 3 (Articolo in rivista) (literal)

Anno

2013-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.3389/fmicb.2013.00082 (literal)

Alternative label

Maree H.J., Almeida R.P.P., Bester R., Chooi K.M., Cohen D., Dolja V.V., Fuchs M.F., Golino D.A., Jooste A.E.C., Martelli G.P., Naidu R.A., Rowhani A., Saldarelli P., Burger J.T. (2013)
Grapevine leafroll-associated virus 3
in Frontiers in microbiology
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Maree H.J., Almeida R.P.P., Bester R., Chooi K.M., Cohen D., Dolja V.V., Fuchs M.F., Golino D.A., Jooste A.E.C., Martelli G.P., Naidu R.A., Rowhani A., Saldarelli P., Burger J.T. (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.scopus.com/inward/record.url?eid=2-s2.0-84884224046&partnerID=q2rCbXpz (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

4 (literal)

Rivista

Frontiers in microbiology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

APR (literal)

Note

Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Department of Genetics, Stellenbosch University, Stellenbosch, South Africa; Biotechnology Platform, Agricultural Research Council, Stellenbosch, South Africa; Department of Environmental Science, Policy and Management, University of California, Berkeley, CA, United States; School of Biological Sciences, University of Auckland, Auckland, New Zealand; The New Zealand Institute for Plant and Food Research, Auckland, New Zealand; Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, United States; Department of Plant Pathology and Plant-Microbe Biology, Cornell University, Geneva, NY, United States; Department of Plant Pathology, University of California, Davis, CA, United States; Plant Protection Research Institute, Agricultural Research Council, Pretoria, South Africa; Department of Soil, Plant and Food Sciences, University Aldo Moro of Bari, Bari, Italy; Department of Plant Pathology, Irrigated Agriculture Research and Extension Center, Washington State University, Prosser, WA, United States; Institute of Plant Virology, National Research Council, Bari, Italy (literal)

Titolo

Grapevine leafroll-associated virus 3 (literal)

Abstract

Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is difficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if intervention strategies are not implemented. Several viruses from the family Closteroviridae are associated with GLD. However, Grapevine leafroll-associated virus 3 (GLRaV-3), the type species for the genus Ampelovirus, is regarded as the most important causative agent. Here we provide a general overview on various aspects of GLRaV-3, with an emphasis on the latest advances in the characterization of the genome. The full genome of several isolates have recently been sequenced and annotated, revealing the existence of several genetic variants. The classification of these variants, based on their genome sequence, will be discussed and a guideline is presented to facilitate future comparative studies. The characterization of sgRNAs produced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs. The latest nucleotide sequence based molecular diagnostic techniques were shown to be more sensitive than conventional serological assays and although ELISA is not as sensitive it remains valuable for high-throughput screening and complementary to molecular diagnostics. The application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction. Next-generation sequencing data can provide information regarding disease complexes, variants of viral species, and abundance of particular viruses. This information can be used to develop more accurate diagnostic assays. Reliable virus screening in support of robust grapevine certification programs remains the cornerstone of GLD management. © 2013 Maree, Almeida, Bester, Chooi, Cohen, Dolja, Fuchs, Golino, Jooste, Martelli, Naidu, Rowhani, Saldarelli and Burger. (literal)
Grapevine leafroll disease (GLD) is one of the most important grapevine viral diseases affecting grapevines worldwide. The impact on vine health, crop yield, and quality is dif- ficult to assess due to a high number of variables, but significant economic losses are consistently reported over the lifespan of a vineyard if intervention strategies are not implemented. Several viruses from the family Closteroviridae are associated with GLD. However, Grapevine leafroll-associated virus 3 (GLRaV-3), the type species for the genus Ampelovirus, is regarded as the most important causative agent. Here we provide a gen- eral overview on various aspects of GLRaV-3, with an emphasis on the latest advances in the characterization of the genome. The full genome of several isolates have recently been sequenced and annotated, revealing the existence of several genetic variants. The classifi- cation of these variants, based on their genome sequence, will be discussed and a guideline is presented to facilitate future comparative studies. The characterization of sgRNAs pro- duced during the infection cycle of GLRaV-3 has given some insight into the replication strategy and the putative functionality of the ORFs. The latest nucleotide sequence based molecular diagnostic techniques were shown to be more sensitive than conventional sero- logical assays and although ELISA is not as sensitive it remains valuable for high-throughput screening and complementary to molecular diagnostics.The application of next-generation sequencing is proving to be a valuable tool to study the complexity of viral infection as well as plant pathogen interaction. Next-generation sequencing data can provide information regarding disease complexes, variants of viral species, and abundance of particular viruses. This information can be used to develop more accurate diagnostic assays. Reliable virus screening in support of robust grapevine certification programs remains the cornerstone of GLD management. (literal)

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