Electrospray ionization multi-stage mass spectrometric study of the interaction products of the cytotoxic complex [Cu(thp)4][PF6] with methionine-rich model peptides (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Electrospray ionization multi-stage mass spectrometric study of the interaction products of the cytotoxic complex [Cu(thp)4][PF6] with methionine-rich model peptides (Articolo in rivista) (literal)

Anno

• 2015-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1002/rcm.7100 (literal)

Alternative label

• Valentina Peruzzo, Francesco Tisato, Marina Porchia, Carlo Santini, Maura Pellei and Piero Traldi (2015)
  Electrospray ionization multi-stage mass spectrometric study of the interaction products of the cytotoxic complex [Cu(thp)4][PF6] with methionine-rich model peptides
  in Rapid communications in mass spectrometry (Online); John Wiley & Sons Ltd., Chichester (Regno Unito)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Valentina Peruzzo, Francesco Tisato, Marina Porchia, Carlo Santini, Maura Pellei and Piero Traldi (literal)

Pagina inizio

• 253 (literal)

Pagina fine

• 262 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://onlinelibrary.wiley.com/doi/10.1002/rcm.7100/pdf (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 29 (literal)

Rivista

• Rapid communications in mass spectrometry (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• 1-3,6 : CNR - IENI, Corso Stati Uniti 4, 35127 Padova, Italy / 4-5 : Scuola di Scienze e Tecnologie, Divisione di Chimica, Università di Camerino, Via S. Agostino 1, I 62032 Camerino, (MC), Italy (literal)

Titolo

• Electrospray ionization multi-stage mass spectrometric study of the interaction products of the cytotoxic complex [Cu(thp)4][PF6] with methionine-rich model peptides (literal)

Abstract

• RATIONALE: The cytotoxic activity of the copper(I) complex [Cu(thp)4][PF6] (CP) (thp = tris(hydroxymethyl) phosphine) is correlated with its high accumulation in cancer cells. Human copper transporter 1 (hCtr1) has been described as the main trans-membrane protein involved in cellular trafficking of physiological copper. Methionine-rich peptide sequences incorporated in the extracellular domain of hCtr1 play a key role in the cellular internalization of copper. We wish to investigate the interaction of CP with model peptides that mimic the extracellular domain of hCtr1. METHODS: The interaction of CP with methionine-rich and methionine-free model peptides has been investigated by electrospray ionization mass spectrometry and the interaction products have been characterized by multiple collisional experiments, using an ion trap mass instrument. RESULTS: The interaction of CP with selected methionine-rich model peptides, Ac-MMMMPMTFK-NH2 (P1) and AcMGMSYMDSK-NH2 (P2), shows that the native copper complex, after sequential loss of phosphines, induces the formation of [Cu(P1)(thp)]+ and [Cu(P1/P2)]+ adducts reasonably by inclusion of the Cu(I) ion in the peptide framework. Collisionally induced fragmentations (MSn) of [Cu(P1/P2)]+ give evidence that the metal is coordinated by the thioetherS of two adjacent methionine residues. Interaction of the same peptides with the isostructural complex [Ag(thp)4] + or AgNO3 yields similar experimental evidence, leading to [Ag(P1/P2)]+. CONCLUSIONS: Methionine sequences incorporated in model peptides are crucial for the recruitment of copper from CP. Such a metal-peptide interaction does not take place when methionine-free Ac-NleGNleSYNleDSK-NH2 (P3) is utilized. A mechanism for tumor cell internalization of CP involving: (i) chemically driven sequential loss of phosphines from the native tetrahedral complex, followed by (ii) transfer of Cu(I) to the methionine-rich sequences typical of the hCtr1 transporter, is proposed. Copyright © 2014 John Wiley & Sons, Ltd (literal)

Editore

• John Wiley & Sons Ltd. (Editore)

Prodotto di

• Progettazione e sintesi di sistemi molecolari inorganici a base di Tecnezio-99 Renio e Rame per applicazioni in radiodiagnostica e terapia. (PM.P01.003.005) (Modulo)
• Institute of Condensed Matter Chemistry and Energy Technologies (ICMATE) (Istituto)

Autore CNR

• VALENTINA PERUZZO (Unità di personale esterno)
• FRANCESCO TISATO (Persona)
• MARINA PORCHIA (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Electrospray ionization multi-stage mass spectrometric study of the interaction products of the cytotoxic complex [Cu(thp)4][PF6] with methionine-rich model peptides" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• MARINA PORCHIA (Unità di personale interno)
• FRANCESCO TISATO (Persona)
• VALENTINA PERUZZO (Unità di personale esterno)
• http://www.cnr.it/ontology/cnr/individuo/unitaDiPersonaleEsterno/ID25618

Prodotto

• Institute of Condensed Matter Chemistry and Energy Technologies (ICMATE) (Istituto)
• Progettazione e sintesi di sistemi molecolari inorganici a base di Tecnezio-99 Renio e Rame per applicazioni in radiodiagnostica e terapia. (PM.P01.003.005) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Rapid communications in mass spectrometry (Rivista)

Editore di

• John Wiley & Sons Ltd. (Editore)

Insieme di parole chiave di

• Parole chiave di "Electrospray ionization multi-stage mass spectrometric study of the interaction products of the cytotoxic complex [Cu(thp)4][PF6] with methionine-rich model peptides" (Insieme di parole chiave)