Rheological and mechanical properties of acellular and cell-laden methacrylated gellan gum hydrogels (Articolo in rivista)

Type
Label
  • Rheological and mechanical properties of acellular and cell-laden methacrylated gellan gum hydrogels (Articolo in rivista) (literal)
Anno
  • 2013-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jbm.a.34650 (literal)
Alternative label
  • Silva-Correia J.; Gloria A.; Oliveira M.B.; Mano J.F.; Oliveira J.M.; Ambrosio L.; Reis R.L. (2013)
    Rheological and mechanical properties of acellular and cell-laden methacrylated gellan gum hydrogels
    in Journal of biomedical materials research. Part A (Online)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Silva-Correia J.; Gloria A.; Oliveira M.B.; Mano J.F.; Oliveira J.M.; Ambrosio L.; Reis R.L. (literal)
Pagina inizio
  • 3438 (literal)
Pagina fine
  • 3446 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.scopus.com/inward/record.url?eid=2-s2.0-84886729786&partnerID=q2rCbXpz (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 101 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo
  • 12 (literal)
Note
  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 3B's Research Group - Biomaterials Biodegradables and Biomimetics, Department of Polymer Engineering, University of Minho, AvePark, Caldas das Taipas, Guimarães, Portugal; ICVS/3B's - PT Government Associate Laboratory, Braga/Guimarães, Portugal; Institute of Composite and Biomedical Materials, National Research Council of Italy, P.le Tecchio 80, 80125 Naples, Italy (literal)
Titolo
  • Rheological and mechanical properties of acellular and cell-laden methacrylated gellan gum hydrogels (literal)
Abstract
  • Tissue engineered hydrogels hold great potential as nucleus pulposus substitutes (NP), as they promote intervertebral disc (IVD) regeneration and re-establish its original function. But, the key to their success in future clinical applications greatly depends on its ability to replicate the native 3D micro-environment and circumvent their limitation in terms of mechanical performance. In the present study, we investigated the rheological/mechanical properties of both ionic- (iGG-MA) and photo-crosslinked methacrylated gellan gum (phGG-MA) hydrogels. Steady shear analysis, injectability and confined compression stress-relaxation tests were carried out. The injectability of the reactive solutions employed for the preparation of iGG-MA and phGG-MA hydrogels was first studied, then the zero-strain compressive modulus and permeability of the acellular hydrogels were evaluated. In addition, human intervertebral disc (hIVD) cells encapsulated in both iGG-MA and phGG-MA hydrogels were cultured in vitro, and its mechanical properties also investigated under dynamic mechanical analysis at 37°C and pH 7.4. After 21 days of culturing, hIVD cells were alive (Calcein AM) and the E? of ionic-crosslinked hydrogels and photo-crosslinked was higher than that observed for acellular hydrogels. Our study suggests that methacrylated gellan gum hydrogels present promising mechanical and biological performance as hIVD cells were producing extracellular matrix. © 2013 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A: 3438-3446, 2013. Copyright © 2013 Wiley Periodicals, Inc., a Wiley Company. (literal)
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