The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27-kDa ?-zein sequence (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27-kDa ?-zein sequence (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.3389/fbioe.2014.00067 (literal)

Alternative label

• Anna Hofbauer, Jenny Peters, Elsa Arcalis, Thomas Rademacher, Johannes Lampel, François Eudes, Alessandro Vitale and Eva Stoger (2014)
  The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27-kDa ?-zein sequence
  in Frontiers in Bioengineering and Biotechnology; Frontiers Research Foundation, Lausanne (Swaziland)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Anna Hofbauer, Jenny Peters, Elsa Arcalis, Thomas Rademacher, Johannes Lampel, François Eudes, Alessandro Vitale and Eva Stoger (literal)

Pagina inizio

• article 67 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://journal.frontiersin.org/Journal/10.3389/fbioe.2014.00067/abstract (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 2 (literal)

Rivista

• Frontiers in Bioengineering and Biotechnology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 10 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• AH, JP, EA, JL, ES: University of Natural Resources and Life Sciences, Vienna, Austria; TR: RWTH Aachen University, Aachen, Germany; FE: Agriculture and Agri-Food Canada, Lethbridge, AB, Canada; AV: IBBA-CNR, Milano (literal)

Titolo

• The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27-kDa ?-zein sequence (literal)

Abstract

• Naturally occurring storage proteins such as zeins are used as fusion partners for recombinant proteins because they induce the formation of ectopic storage organelles known as protein bodies (PBs) where the proteins are stabilized by intermolecular interactions and the formation of disulfide bonds. Endogenous PBs are derived from the endoplasmic reticulum (ER). Here, we have used different targeting sequences to determine whether ectopic PBs composed of the N-terminal portion of mature 27 kDa ?-zein added to a fluorescent protein could be induced to form elsewhere in the cell. The addition of a transit peptide for targeting to plastids causes PB formation in the stroma, whereas in the absence of any added targeting sequence PBs were typically associated with the plastid envelope, revealing the presence of a cryptic plastid-targeting signal within the ?-zein cysteine-rich domain. The subcellular localization of the PBs influences their morphology and the solubility of the stored recombinant fusion protein. Our results indicate that the biogenesis and budding of PBs does not require ER-specific factors and therefore, confirm that ?-zein is a versatile fusion partner for recombinant proteins offering unique opportunities for the accumulation and bioencapsulation of recombinant proteins in different subcellular compartments. (literal)

Editore

• Frontiers Research Foundation (Editore)

Prodotto di

• Basi molecolari, fisiologiche e cellulari delle produzioni vegetali. (AG.P01.001.001) (Modulo)
• Institute of agricultural biology and biotechnology (IBBA) (Istituto)

Autore CNR

• ALESSANDRO VITALE (Unità di personale interno)

Insieme di parole chiave

• Keywords of "The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27-kDa ?-zein sequence" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute of agricultural biology and biotechnology (IBBA) (Istituto)
• Basi molecolari, fisiologiche e cellulari delle produzioni vegetali. (AG.P01.001.001) (Modulo)

Autore CNR di

• ALESSANDRO VITALE (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Frontiers in Bioengineering and Biotechnology (Rivista)

Editore di

• Frontiers Research Foundation (Editore)

Insieme di parole chiave di

• Keywords of "The induction of recombinant protein bodies in different subcellular compartments reveals a cryptic plastid-targeting signal in the 27-kDa ?-zein sequence" (Insieme di parole chiave)