Scope and limitations of the TEMPO/EPR method for singlet oxygen detection: The misleading role of electron transfer (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Scope and limitations of the TEMPO/EPR method for singlet oxygen detection: The misleading role of electron transfer (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.freeradbiomed.2014.08.020 (literal)

Alternative label

• Nardi G.; Manet I.; Monti S.; Miranda M.A.; Lhiaubet-Vallet V. (2014)
  Scope and limitations of the TEMPO/EPR method for singlet oxygen detection: The misleading role of electron transfer
  in Free radical biology & medicine
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Nardi G.; Manet I.; Monti S.; Miranda M.A.; Lhiaubet-Vallet V. (literal)

Pagina inizio

• 64 (literal)

Pagina fine

• 70 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.scopus.com/inward/record.url?eid=2-s2.0-84908418868&partnerID=q2rCbXpz (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 77 (literal)

Rivista

• Free radical biology & medicine (Rivista)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Instituto de Tecnología Química UPV-CSIC, Universitat Politècnica de València, Valencia, 46022, Spain; Istituto Per la Sintesi Organica e la Fotoreattività, Consiglio Nazionale Delle Ricerche, Bologna, 40129, Italy (literal)

Titolo

• Scope and limitations of the TEMPO/EPR method for singlet oxygen detection: The misleading role of electron transfer (literal)

Abstract

• For many biological and biomedical studies, it is essential to detect the production of 1 O2 and quantify its production yield. Among the available methods, detection of the characteristic 1270-nm phosphorescence of singlet oxygen by time-resolved near-infrared (TRNIR) emission constitutes the most direct and unambiguous approach. An alternative indirect method is electron paramagnetic resonance (EPR) in combination with a singlet oxygen probe. This is based on the detection of the TEMPO free radical formed after oxidation of TEMP (2,2,6,6-tetramethylpiperidine) by singlet oxygen. Although the TEMPO/EPR method has been widely employed, it can produce misleading data. This is demonstrated by the present study, in which the quantum yields of singlet oxygen formation obtained by TRNIR emission and by the TEMPO/EPR method are compared for a set of well-known photosensitizers. The results reveal that the TEMPO/EPR method leads to significant overestimation of singlet oxygen yield when the singlet or triplet excited state of the photosensitizer is efficiently quenched by TEMP, acting as electron donor. In such case, generation of the TEMP+. radical cation, followed by deprotonation and reaction with molecular oxygen, gives rise to an EPR-detectable TEMPO signal that is not associated with singlet oxygen production. This knowledge is essential for an appropriate and error-free application of the TEMPO/EPR method in chemical, biological, and medical studies. (literal)

Prodotto di

• Studio delle interazioni legando-recettore in sistemi modello mediante metodi spettroscopici e fotofisici (LEGARE) (PM.P06.005.002) (Modulo)
• Institute for organic syntheses and photoreactivity (ISOF) (Istituto)

Autore CNR

• ILSE GERT MANET (Unità di personale interno)
• SANDRA MONTI (Unità di personale esterno)

Insieme di parole chiave

• Keywords of "Scope and limitations of the TEMPO/EPR method for singlet oxygen detection: The misleading role of electron transfer" (Insieme di parole chiave)

Incoming links:

Prodotto

• Institute for organic syntheses and photoreactivity (ISOF) (Istituto)
• Studio delle interazioni legando-recettore in sistemi modello mediante metodi spettroscopici e fotofisici (LEGARE) (PM.P06.005.002) (Modulo)

Autore CNR di

• ILSE GERT MANET (Unità di personale interno)
• SANDRA MONTI (Unità di personale esterno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Free radical biology & medicine (Rivista)

Insieme di parole chiave di

• Keywords of "Scope and limitations of the TEMPO/EPR method for singlet oxygen detection: The misleading role of electron transfer" (Insieme di parole chiave)