Metal ions modulate thermal aggregation of beta-lactoglobulin: a join chemical and physical characterisation (Articolo in rivista)

Type
Label
  • Metal ions modulate thermal aggregation of beta-lactoglobulin: a join chemical and physical characterisation (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Navarra G, Tinti A, Di Foggia M, Leone M, Militello V, Torreggiani A (2014)
    Metal ions modulate thermal aggregation of beta-lactoglobulin: a join chemical and physical characterisation
    in Journal of inorganic biochemistry
    (literal)
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  • Navarra G, Tinti A, Di Foggia M, Leone M, Militello V, Torreggiani A (literal)
Pagina inizio
  • 64 (literal)
Pagina fine
  • 73 (literal)
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  • 137 (literal)
Rivista
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  • Dipartimento di Fisica-Chimica, Università di Palermo, Via Archirafi 36, 90123 Palermo, Italy Dipartimento di Scienze Biomediche e Neuromotorie, Università di Bologna, Via Belmeloro 8/2, 40126 Bologna, Italy Istituto I.S.O.F., Consiglio Nazionale delle Ricerche, Via P. Gobetti 101, 40129 Bologna, Italy (literal)
Titolo
  • Metal ions modulate thermal aggregation of beta-lactoglobulin: a join chemical and physical characterisation (literal)
Abstract
  • Molecular basis of the role played by Cu(2+) and Zn(2+) ions during the thermal aggregation processes of beta-lactoglobulin (BLG) was studied by using a joint application of different techniques. In particular, Raman spectroscopy was very useful in identifying the different effects caused by the two metals at molecular level (i.e. changes in His protonation state, disulfides bridge conformation, and micro-environment of aromatic residues), evidencing the primary importance of the protein charge distribution during the aggregation process. Both metal ions are able to act on this factor and favor the protein aggregation, but Zn(2+) is able to alter the natural conformational state of BLG, causing a slight unfolding, whereas Cu(2+) ions play a role only during the thermal treatment. Thus, Zn(2+) ions favor the formation of bigger aggregates and branched fibril-like structures, whereas for Cu(2+) ions a greater number of cross-beta structures during thermal incubation and finally, fibrillar structures. The aggregation process occurs in two phases, as suggested by the measurements on the time evolution of the BLG aggregates: the first one is characterized by a partial unfolding of the protein and aggregate growth, forming oligomers and protofibrils, whereas the second one is characterized by further supramolecular assembly, leading to the formation of fibrils. (literal)
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