Imidazole-stabilized gold nanoparticles induce neuronal apoptosis: An in vitro and in vivo study (Articolo in rivista)

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  • Imidazole-stabilized gold nanoparticles induce neuronal apoptosis: An in vitro and in vivo study (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1002/jbm.a.35289 (literal)
Alternative label
  • Imperatore, Roberta; Carotenuto, Gianfranco; Di Grazia, Maria Antonietta; Ferrandino, Ida; Palomba, Letizia; Vitale, Emilia; Mariotti, Raffaella; De Nicola, Sergio; Longo, Angela; Cristino, Luigia (2014)
    Imidazole-stabilized gold nanoparticles induce neuronal apoptosis: An in vitro and in vivo study
    in Journal of biomedical materials research. Part A
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Imperatore, Roberta; Carotenuto, Gianfranco; Di Grazia, Maria Antonietta; Ferrandino, Ida; Palomba, Letizia; Vitale, Emilia; Mariotti, Raffaella; De Nicola, Sergio; Longo, Angela; Cristino, Luigia (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://www.scopus.com/record/display.url?eid=2-s2.0-84905313395&origin=inward (literal)
Rivista
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  • Scopu (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Imperatore, Roberta, Di Grazia, Maria Antonietta, Cristino, Luigia: Institute of Biomolecular Chemistry, CNR of Pozzuoli, Naples, Italy Carotenuto, Gianfranco, Longo, Angela: 2Institute for Composite and Biomedical Materials, CNR of Naples, Naples, Italy Ferrandino, Ida: Department of Biological Sciences, University of Naples Federico II, Naples, Italy Palomba, Letizia: Department of Biomolecular Sciences, University of Urbino \"Carlo Bo\", Urbino, Italy Mariotti, Raffaella: Department of Neurological and Movement Sciences, University of Verona, Verona, Italy Viatale Emilia: Institute of Protein Biochemistry, CNR of Naples, Naples, Italy De Nicola Sergio: SPIN Institute, National Research Council, Complesso Universitario of M.S. Angelo, Naples, Italy (literal)
Titolo
  • Imidazole-stabilized gold nanoparticles induce neuronal apoptosis: An in vitro and in vivo study (literal)
Abstract
  • Gold nanoparticles are increasingly being employed in innovative biological applications thanks to their advantages of material- and size-dependent physics and chemical interactions with the cellular systems. On the other hand, growing concern has emerged on the toxicity which would render gold-based nanoparticles harmful to cell cultures, animals, and humans. Emerging attention is focused on the interaction of gold nanoparticles with nervous system, especially regarding the ability to overcome the blood-brain barrier (BBB) which represents the major impediment to the delivery of therapeutics into the brain. We synthesized highly stable 2-mercapto-1-methylimidazole-stabilized gold-nanoparticles (AuNPs)-mmi to investigate their entry, accumulation, and toxicity in vitro (SH-SY5Y human neuroblastoma cells) and in vivo (brain of C57BL/6 mice) through optical and electron microscopy. After incubation in the cell culture medium at the lowest dose of 0.1 mg/mL the (AuNPs)-mmi nanoparticles were found compacted and recruited into endosome/lysosomes (1 h) before their fusion (2 h) and the onset of neuronal death by apoptosis (4 h) as proved by terminal-transferase-mediated dUTP nick end labeling assay and caspase-3 immunoreactivity. The ability of (AuNPs)-mmi to cross the BBB was assessed by injection in the caudal vein of C57BL/6 mice. Among different brain regions, the nanoparticles were found in the CaudatoPutamen area, mainly in the striatal neurons 4 h after injection. These neurons showed the typical hallmarks of apoptosis. Our findings provide, for the first time, the dynamic of 2-mercapto-1-methylimidazole nanogold uptake. The molecular mechanism which underlies the nanogold-driven apoptotic event is analyzed and discussed in order to take into account when designing nanomaterials to interface with biological structures. © 2014 Wiley Periodicals, Inc. (literal)
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