Genotyping of Staphylococcus aureus from clinical mastitis in a commercial dairy farm (Abstract/Poster in atti di convegno)

Type
Label
  • Genotyping of Staphylococcus aureus from clinical mastitis in a commercial dairy farm (Abstract/Poster in atti di convegno) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Alternative label
  • Martina Besozzi1, Clara Locatelli1, Claudia Pollera1, Camilla Luzzago1, Paola Cremonesi2, Bianca Castiglioni2, Nicola Rota1, Antonio Casula1, Roger Guix3, Valerio Bronzo1, Paolo Moroni*1 (2014)
    Genotyping of Staphylococcus aureus from clinical mastitis in a commercial dairy farm
    in National Mastitis Council Regional Meeting 2014, Ghent, August, 4-5-6
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Martina Besozzi1, Clara Locatelli1, Claudia Pollera1, Camilla Luzzago1, Paola Cremonesi2, Bianca Castiglioni2, Nicola Rota1, Antonio Casula1, Roger Guix3, Valerio Bronzo1, Paolo Moroni*1 (literal)
Note
  • Abstract (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • 1 Università degli Studi di Milano, Milan, Italy 2 Istituto di Biologia e Biotecnologia Agraria, Consiglio Nazionale delle Ricerche, Lodi, Italy 3 Hipra, Girona, Spain (literal)
Titolo
  • Genotyping of Staphylococcus aureus from clinical mastitis in a commercial dairy farm (literal)
Abstract
  • The epidemiology of intramammary infections is specific of each farm as well as the distribution of pathogens causing clinical mastitis. Staphylococcus aureus is an important contagious pathogen that diffuses throughout a herd essentially during milking. However, new infections can occur depending on strains specificities and management. Milk samples from clinical mastitis were collected in one commercial dairy farm for twenty-two months and cultured according to NMC guidelines. The farm consisted of 150 lactating cows. S. aureus isolates were genotyped by 16-23S rRNA intergenic spacer region (RSPCR) and characterized by PCR for detection of a panel of virulence factors. A total of 96 isolates were tested. In spite of the different phenotypes at culture, one genotype (GTB) resulted prevalent (77/96), 17 isolates belonged to a subtype of GTB and two isolates to a novel genotype. All the isolates were positive for enterotoxins A, D and J, and negative for Panton Valentine Leukocidin, mecA gene and Toxic shock syndrome toxin. The results of RSPCR on this farm confirm the diffusion of a restricted number of strains, consistent with contagiousness, and a notable prevalence of GTB. The virulence patterns are typical of bovine strains. (literal)
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