Nano- and femtosecond UV laser pulses to immobilize biomolecules onto surfaces with preferential orientation (Articolo in rivista)

Type
Label
  • Nano- and femtosecond UV laser pulses to immobilize biomolecules onto surfaces with preferential orientation (Articolo in rivista) (literal)
Anno
  • 2014-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1007/s00339-014-8340-4 (literal)
Alternative label
  • Lettieri, S.; Avitabile, A.; Della Ventura, B.; Funari, R.; Ambrosio, A.; Maddalena, P.; Valadan, M.; Velotta, R.; Altucci, C. (2014)
    Nano- and femtosecond UV laser pulses to immobilize biomolecules onto surfaces with preferential orientation
    in Applied physics. A, Materials science & processing (Print)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Lettieri, S.; Avitabile, A.; Della Ventura, B.; Funari, R.; Ambrosio, A.; Maddalena, P.; Valadan, M.; Velotta, R.; Altucci, C. (literal)
Pagina inizio
  • 185 (literal)
Pagina fine
  • 190 (literal)
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  • 117 (literal)
Rivista
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  • 6 (literal)
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  • 1 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Dipartimento di Fisica, Università degli Studi di Napoli Federico II, Complesso Universitario Monte S. Angelo, Via Cintia, Naples, 80126, Italy CNR-SPIN, UOS Napoli, Complesso Universitario Monte S. Angelo, Via Cintia, Naples, 80126, Italy Dipartimento di Fisica, Università di Roma, La Sapienza, Rome, Italy Consorzio Nazionale Interuniversitario per le Scienze Fisiche della Materia, Naples, Italy (literal)
Titolo
  • Nano- and femtosecond UV laser pulses to immobilize biomolecules onto surfaces with preferential orientation (literal)
Abstract
  • By relying on the photonic immobilization technique of antibodies onto surfaces, we realized portable biosensors for light molecules based on the use of quartz crystal microbalances, given the linear dependence of the method on the laser pulse intensity. Here, we compare the quality of the anchoring method when using nanosecond (260 nm, 25 mJ/pulse, 5 ns, 10 Hz rep. rate) and femtosecond (258 nm, 25 mu J/pulse, 150 fs, 10 kHz rep. rate) laser source, delivering the same energy to the sample with the same average power. As a reference, we also tethered untreated antibodies by means of the passive adsorption. The results are striking: When the antibodies are irradiated with the femtosecond pulses, the deposition on the gold plate is much more ordered than in the other two cases. The effects of UV pulses irradiation onto the antibodies are also analyzed by measuring absorption and fluorescence and suggest the occurrence of remarkable degradation when nanosecond pulses are used likely induced by a larger thermal coupling. In view of the high average power required to activate the antibodies for the achievement of the photonic immobilization technique, we conclude that femtosecond rather than nanosecond laser pulses have to be used. (literal)
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