Enhancement of the Uptake and Cytotoxic Activity of Doxorubicin in Cancer Cells by Novel cRGD-Semipeptide-Anchoring Liposomes (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Enhancement of the Uptake and Cytotoxic Activity of Doxorubicin in Cancer Cells by Novel cRGD-Semipeptide-Anchoring Liposomes (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1021/mp400718j (literal)

Alternative label

• Battistini, Lucia; Burreddu, Paola; Sartori, Andrea; Arosio, Daniela; Manzoni, Leonardo; Paduano, Luigi; D'Errico, Gerardino; Sala, Roberto; Reia, Laura; Bonomini, Sabrina; Rassu, Gloria; Zanardi, Franca (2014)
  Enhancement of the Uptake and Cytotoxic Activity of Doxorubicin in Cancer Cells by Novel cRGD-Semipeptide-Anchoring Liposomes
  in Molecular pharmaceutics (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Battistini, Lucia; Burreddu, Paola; Sartori, Andrea; Arosio, Daniela; Manzoni, Leonardo; Paduano, Luigi; D'Errico, Gerardino; Sala, Roberto; Reia, Laura; Bonomini, Sabrina; Rassu, Gloria; Zanardi, Franca (literal)

Pagina inizio

• 2280 (literal)

Pagina fine

• 2293 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 11 (literal)

Rivista

• Molecular pharmaceutics (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali

• 14 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 7 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• University of Parma; Consiglio Nazionale delle Ricerche (CNR); Consiglio Nazionale delle Ricerche (CNR); University of Naples Federico II; CSGI Consorzio Interuniv Sviluppo Sistemi Grande; University of Parma; University of Parma (literal)

Titolo

• Enhancement of the Uptake and Cytotoxic Activity of Doxorubicin in Cancer Cells by Novel cRGD-Semipeptide-Anchoring Liposomes (literal)

Abstract

• Novel liposemipeptides hanging cyclic azabicycloalkane-RGD or aminoproline-RGD terminals were synthesized and incorporated into liposomal nanoparticles cAba/cAmpRGD-LNP5 3C/3D. Liposomes with similar composition and lacking semipeptide conjugates were constructed for comparison (LNP, 3A), and physical encapsulation of the anticancer doxorubicin drug in both targeted and untargeted liposomes was accomplished. Microstructural analysis performed by dynamic light scattering (DLS), small-angle neutron scattering (SANS), and electron paramagnetic resonance (EPR) revealed that the conjugated nanoparticles presented an average size of 80 nm and were constituted by 5 nm thick unilamellar liposome bilayer. Flow cytometry and fluorescent microscopy studies showed that 3C-DOXO and 3D-DOXO efficiently delivered the drug into the nuclei of both quiescent and proliferating cells even in a high serum concentration environment. The uptake of doxorubicin when carried by liposomes was faster than that of the free drug, and 30 min incubation was sufficient to load cell nuclei with doxorubicin. Targeted liposomes significantly induced cell death of human breast adenocarcinoma MCF7 cells (IC50 = 144 nM, 3C-DOXO; IC50 = 274 nM, 3D-DOXO), about 2- to 6-fold more potent than free doxorubicin or 3A-DOXO controls (IC50 = 527 and 854 nM, respectively). These results suggest that cAba/cAmpRGD liposomal nanoparticles hold promise for the rapid and efficient delivery of chemotherapeutic agents to alpha(V)beta(3)-expressing tumor cells. (literal)

Prodotto di

• Istituto di chimica biomolecolare (ICB) (Istituto)
• Ligandi integrinici per la diagnostica e terapia tumorale (PM.P01.021.005) (Modulo)
• Istituto di scienze e tecnologie molecolari (ISTM) (Istituto)

Autore CNR

• DANIELA AROSIO (Unità di personale interno)
• LEONARDO PIERPAOLO MANZONI (Unità di personale interno)
• GLORIA MARIA RITA RASSU (Unità di personale interno)
• PAOLA BURREDDU (Unità di personale interno)

Insieme di parole chiave

• Keywords of "Enhancement of the Uptake and Cytotoxic Activity of Doxorubicin in Cancer Cells by Novel cRGD-Semipeptide-Anchoring Liposomes" (Insieme di parole chiave)

Incoming links:

Prodotto

• Istituto di scienze e tecnologie molecolari (ISTM) (Istituto)
• Istituto di chimica biomolecolare (ICB) (Istituto)
• Ligandi integrinici per la diagnostica e terapia tumorale (PM.P01.021.005) (Modulo)

Autore CNR di

• LEONARDO PIERPAOLO MANZONI (Unità di personale interno)
• GLORIA MARIA RITA RASSU (Unità di personale interno)
• DANIELA AROSIO (Unità di personale interno)
• PAOLA BURREDDU (Unità di personale interno)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular pharmaceutics (Rivista)

Insieme di parole chiave di

• Keywords of "Enhancement of the Uptake and Cytotoxic Activity of Doxorubicin in Cancer Cells by Novel cRGD-Semipeptide-Anchoring Liposomes" (Insieme di parole chiave)