http://www.cnr.it/ontology/cnr/individuo/prodotto/ID285540

Short-term Type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver (Articolo in rivista)

Type

Articolo in rivista (Classe)
Prodotto della ricerca (Classe)

Label

Short-term Type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver (Articolo in rivista) (literal)

Anno

2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

10.1111/eci.12241 (literal)

Alternative label

Taurino F.; Stanca E.; Vonghia L.; Siculella L.; Sardanelli A.M.; Papa S.; Zanotti F.; Gnoni A. (2014)
Short-term Type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver
in European journal of clinical investigation (Print)
(literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

Taurino F.; Stanca E.; Vonghia L.; Siculella L.; Sardanelli A.M.; Papa S.; Zanotti F.; Gnoni A. (literal)

Pagina inizio

350 (literal)

Pagina fine

358 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

http://www.scopus.com/inward/record.url?eid=2-s2.0-84895930382&partnerID=q2rCbXpz (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

44 (literal)

Rivista

European journal of clinical investigation (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

4 (literal)

Note

Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

Department of Basical Medical Sciences, Neurosciences and Sensory Organs, University of Bari Aldo Moro, Bari, Italy; Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy; Institution of Translational Pharmacology, National Research Council, Rome, Italy; Institution of Biomembranes and Bioenergetics, CNR, Bari, Italy (literal)

Titolo

Short-term Type-1 diabetes differentially modulates 14-3-3 proteins in rat brain and liver (literal)

Abstract

Background: The 14-3-3 proteins family consists of seven proteins that are highly conserved molecular chaperones with roles in the regulation of metabolism, signal transduction, cell cycle control, protein trafficking and apoptosis. Their role in several pathologies has been reported. In this study, we investigated the mRNA and protein expression of the 14-3-3s in rat brain and liver in the early stage of Type-1 diabetes (T1D). Material and methods: Diabetes was induced by a single intraperitoneal injection (70 mg/kg bw) of freshly prepared streptozotocin (STZ), and, after 3 weeks of treatment, brain and liver nuclei and cytosolic extracts were prepared. Quantitative real-time PCR and Western blotting analyses were performed to evaluate mRNA and protein expression for each of the seven 14-3-3s. Results: In nondiabetic control rats, the expression profile of 14-3-3s revealed a tissue-specific distribution, and the expression level of each isoform was found higher in the brain than in the liver. In the diabetic brain, mRNA and protein levels of the 14-3-3?, ?, ?, ? and ? were lower; 14-3-3? mRNA significantly increased while its protein level decreased. In the diabetic liver, the mRNA of 14-3-3?, 14-3-3? and 14-3-3? significantly increased, but only the 14-3-3? protein level increased. Overall, in diabetic animals, the changes in the expression levels of brain 14-3-3s were much more pronounced than in the liver. Conclusion: Our results indicate that during the early phase of STZ-induced T1D, the 14-3-3 proteins are affected in an isoform- and tissue-specific way. © 2014 Stichting European Society for Clinical Investigation Journal Foundation. (literal)

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