Multiple IgE recognition on the major allergen of the Parietaria pollen Par j 2 (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Multiple IgE recognition on the major allergen of the Parietaria pollen Par j 2 (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.molimm.2014.09.012 (literal)

Alternative label

• Valeria Longo, Maria Assunta Costa, Fabio Cibella, Giuseppina Cuttitta, Stefania La Grutta, Paolo Colombo (2014)
  Multiple IgE recognition on the major allergen of the Parietaria pollen Par j 2
  in Molecular immunology
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Valeria Longo, Maria Assunta Costa, Fabio Cibella, Giuseppina Cuttitta, Stefania La Grutta, Paolo Colombo (literal)

Rivista

• Molecular immunology (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• IBIM - Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (literal)

Titolo

• Multiple IgE recognition on the major allergen of the Parietaria pollen Par j 2 (literal)

Abstract

• The interaction between IgE antibodies and allergens is a key event in triggering an allergic reaction. The characterization of this region provides information of paramount importance for diagnosis and therapy. Par j 2 Lipid Transfer Protein is one of the most important allergens in southern Europe and a wellestablished marker of sensitization in Parietaria pollen allergy. The main aim of this study was to map the IgE binding regions of this allergen and to study the pattern of reactivity of individual Parietaria-allergic patients. By means of gene fragmentation, six overlapping peptides were expressed in Escherichia coli, and their IgE binding activity was evaluated by immunoblotting in a cohort of 79 Parietaria-allergic patients. Our results showed that Pj-allergic patients display a heterogeneous pattern of IgE binding to the different recombinant fragments, and that patients reacted simultaneously against several protein domains spread all the over the molecule, even in fragments which do not contain structural features resembling the native allergen. Our results reveal the presence of a large number of linear and conformational epitopes on the Par j 2 sequence, which probably explains the high allergenic activity of this allergen (literal)

Prodotto di

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Biotecnologie Molecolari per la Caratterizzazione di Farmaci Innovativi (SV.P17.008.004) (Modulo)
• Epidemiologia delle broncopneumopatie e di altre malattie croniche non comunicabili (ME.P01.014.001) (Modulo)

Autore CNR

• FABIO CIBELLA (Persona)
• PAOLO COLOMBO (Persona)
• STEFANIA LA GRUTTA (Unità di personale interno)
• GIUSEPPINA CUTTITTA (Persona)
• MARIA ASSUNTA COSTA (Unità di personale interno)

Incoming links:

Autore CNR di

• PAOLO COLOMBO (Persona)
• GIUSEPPINA CUTTITTA (Persona)
• FABIO CIBELLA (Persona)
• MARIA ASSUNTA COSTA (Unità di personale interno)
• STEFANIA LA GRUTTA (Unità di personale interno)

Prodotto

• Istituto di biomedicina e di immunologia molecolare \"Alberto Monroy\" (IBIM) (Istituto)
• Epidemiologia delle broncopneumopatie e di altre malattie croniche non comunicabili (ME.P01.014.001) (Modulo)
• Biotecnologie Molecolari per la Caratterizzazione di Farmaci Innovativi (SV.P17.008.004) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Molecular immunology (Rivista)