Quantitative detection of Listeria monocytogenes in raw milk and soft cheeses: Culture-independent versus liquid- and solid-based culture-dependent real time PCR approaches (Articolo in rivista)

Type

• Articolo in rivista (Classe)
• Prodotto della ricerca (Classe)

Label

• Quantitative detection of Listeria monocytogenes in raw milk and soft cheeses: Culture-independent versus liquid- and solid-based culture-dependent real time PCR approaches (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.lwt.2014.03.005 (literal)

Alternative label

• Quero G.M.; Santovito E.; Visconti A.; Fusco V. (2014)
  Quantitative detection of Listeria monocytogenes in raw milk and soft cheeses: Culture-independent versus liquid- and solid-based culture-dependent real time PCR approaches
  in Lebensmittel-Wissenschaft + Technologie
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Quero G.M.; Santovito E.; Visconti A.; Fusco V. (literal)

Pagina inizio

• 11 (literal)

Pagina fine

• 20 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.scopus.com/inward/record.url?eid=2-s2.0-84898787782&partnerID=q2rCbXpz (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 58 (literal)

Rivista

• Lebensmittel-Wissenschaft + Technologie (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 1 (literal)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• National Research Council of Italy, Institute of Sciences of Food Production (CNR-ISPA), Via Amendola 122/O, 70126 Bari, Italy (literal)

Titolo

• Quantitative detection of Listeria monocytogenes in raw milk and soft cheeses: Culture-independent versus liquid- and solid-based culture-dependent real time PCR approaches (literal)

Abstract

• A culture-independent and two culture-dependent real time (rt-) PCR approaches were developed to quantitatively identify Listeria monocytogenes in raw milk and soft cheeses. The optimised rt-PCR revealed 100% inclusivity and exclusivity. DNA- and cell-based standard curves showed a good linearity of response (R2 ? 0.987 and R2 ? 0.998, respectively) for five orders of magnitude (39 ? 105 e 3 ? 100 genome equivalents and 106e101 CFU equivalents, respectively) with about 100% relative accuracy and inter-assay variability ?0.90%. Up to 1 genome equivalent/and 10 CFU/reaction were quantified in the DNA and cell standard curves, respectively. The rt-PCR was then combined with a liquid- (MPN technique) or a solid- (ALOA and PALCAM) based enumeration. The diagnostic sensitivity of the different approaches was investigated in artificially contaminated raw milk and soft cheeses. The rt-PCR culture-independent approach performed well in raw milk and (with a lower sensitivity) in stracchino cheese-based standard curves. MPN/rt-PCR was the best approach to enumerate low levels of L. monocytogenes in raw milk and stracchino cheese, while the ALOA-based rt-PCR quantification was more effective than the PALCAM-based. These performances were confirmed when 23 real samples of raw milk and soft cheeses by both the rt-PCR approaches were assayed. (literal)

Prodotto di

• Microbiologia e qualità delle produzioni alimentari (AG.P05.016.001) (Modulo)
• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)

Autore CNR

• GRAZIA MARINA QUERO (Unità di personale esterno)
• VINCENZINA FUSCO (Unità di personale interno)
• ANGELO VISCONTI (Unità di personale interno)

Insieme di parole chiave

• Parole chiave di "Quantitative detection of Listeria monocytogenes in raw milk and soft cheeses: Culture-independent versus liquid- and solid-based culture-dependent real time PCR approaches" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• ANGELO VISCONTI (Unità di personale interno)
• VINCENZINA FUSCO (Unità di personale interno)
• GRAZIA MARINA QUERO (Unità di personale esterno)
• http://www.cnr.it/ontology/cnr/individuo/unitaDiPersonaleEsterno/ID23342

Prodotto

• Istituto di scienze delle produzioni alimentari (ISPA) (Istituto)
• Microbiologia e qualità delle produzioni alimentari (AG.P05.016.001) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Lebensmittel-Wissenschaft + Technologie (Rivista)

Insieme di parole chiave di

• Parole chiave di "Quantitative detection of Listeria monocytogenes in raw milk and soft cheeses: Culture-independent versus liquid- and solid-based culture-dependent real time PCR approaches" (Insieme di parole chiave)