Reorganization of enhancer patterns in transition from naive to primed pluripotency. (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• Reorganization of enhancer patterns in transition from naive to primed pluripotency. (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.stem.2014.04.003 (literal)

Alternative label

• Buecker, Christa; Srinivasan, Rajini; Wu, Zhixiang; Calo, Eliezer; Acampora, Dario; Faial, Tiago; Simeone, Antonio; Tan, Minjia; Swigut, Tomasz; Wysocka, Joanna (2014)
  Reorganization of enhancer patterns in transition from naive to primed pluripotency.
  in Cell stem cell (Print)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Buecker, Christa; Srinivasan, Rajini; Wu, Zhixiang; Calo, Eliezer; Acampora, Dario; Faial, Tiago; Simeone, Antonio; Tan, Minjia; Swigut, Tomasz; Wysocka, Joanna (literal)

Pagina inizio

• 838 (literal)

Pagina fine

• 53 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 14 (literal)

Rivista

• Cell stem cell (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 6 (literal)

Note

• ISI Web of Science (WOS) (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• Department of Chemical and Systems Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. State Key Laboratory of Drug Research, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China. Institute of Genetics and Biophysics \"Adriano Buzzati-Traverso,\" CNR, Via P. Castellino, 111, 80131 Naples, Italy; IRCCS Neuromed, 86077 Pozzilli (IS), Italy. Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA. (literal)

Titolo

• Reorganization of enhancer patterns in transition from naive to primed pluripotency. (literal)

Abstract

• Naive and primed pluripotency is characterized by distinct signaling requirements, transcriptomes, and developmental properties, but both cellular states share key transcriptional regulators: Oct4, Sox2, and Nanog. Here, we demonstrate that transition between these two pluripotent states is associated with widespread Oct4 relocalization, mirrored by global rearrangement of enhancer chromatin landscapes. Our genomic and biochemical analyses identified candidate mediators of primed state-specific Oct4 binding, including Otx2 and Zic2/3. Even when differentiation cues are blocked, premature Otx2 overexpression is sufficient to exit the naive state, induce transcription of a substantial subset of primed pluripotency-associated genes, and redirect Oct4 to previously inaccessible enhancer sites. However, the ability of Otx2 to engage new enhancer regions is determined by its levels, cis-encoded properties of the sites, and the signaling environment. Our results illuminate regulatory mechanisms underlying pluripotency and suggest that the capacity of transcription factors such as Otx2 and Oct4 to pioneer new enhancer sites is highly context dependent. (literal)

Prodotto di

• Meccanismi molecolari e cellulari della determinazione neurale, differenziamento neuronale e patologie del sistema nervoso (SV.P16.006.001) (Modulo)
• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)

Autore CNR

• ANTONIO SIMEONE (Persona)
• DARIO ACAMPORA (Persona)

Incoming links:

Prodotto

• Istituto di genetica e biofisica \"Adriano Buzzati Traverso\" (IGB) (Istituto)
• Meccanismi molecolari e cellulari della determinazione neurale, differenziamento neuronale e patologie del sistema nervoso (SV.P16.006.001) (Modulo)

Autore CNR di

• ANTONIO SIMEONE (Persona)
• DARIO ACAMPORA (Persona)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Cell stem cell (Rivista)