Isolation, characterization and expression of an elongation factor 1± gene in potato (Solanum tuberosum) cell cultures (Articolo in rivista)

Type
Label
  • Isolation, characterization and expression of an elongation factor 1± gene in potato (Solanum tuberosum) cell cultures (Articolo in rivista) (literal)
Anno
  • 2010-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1080/11263504.2010.489335 (literal)
Alternative label
  • Costa A., Di Giacomo M., Massarelli I., De Palma M., Leone A., Grillo S. (2010)
    Isolation, characterization and expression of an elongation factor 1± gene in potato (Solanum tuberosum) cell cultures
    in Plant Biosystems (Firenze, Testo stamp.)
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • Costa A., Di Giacomo M., Massarelli I., De Palma M., Leone A., Grillo S. (literal)
Pagina inizio
  • 618 (literal)
Pagina fine
  • 625 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
  • 144 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • (ISSN: 1126-3504) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#pagineTotali
  • 8 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Istituo di Genetica vegetale (literal)
Titolo
  • Isolation, characterization and expression of an elongation factor 1± gene in potato (Solanum tuberosum) cell cultures (literal)
Abstract
  • Using a differential display (DDRT-PCR) approach to screen genes involved in response to osmotic stress, we isolated six cDNA clones from potato cell cultures. One of these, clone #54, is a member of the gene family encoding the -subunit of translation elongation factor 1 (EF-1). After sequencing, this clone showed a deduced amino acid sequence of 447 residues. Southern blot analysis revealed that this EF-1 is a member of a family consisting of at least nine genes. The expression of EF-1 increased upon osmotic stress mediated by polyethylene glycol (PEG) but was unaffected by abscisic acid (ABA) treatment. Salt also increased EF-1 expression but with a different kinetics. These results indicate that we cloned an EF-1 in potato which is ABA responsive, independent of osmotic stress. Abbreviations: DDRT-PCR, differential display reverse transcriptase-polymerase chain reaction; PEG, polyethylene glycol; RACE, rapid amplification of cDNA ends; RT-PCR, reverse transcriptase-polymerase chain reaction. (literal)
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