A practical approach for the detection of DNA nanostructures in single live human cells by fluorescence microscopy (Articolo in rivista)

Type

• Prodotto della ricerca (Classe)
• Articolo in rivista (Classe)

Label

• A practical approach for the detection of DNA nanostructures in single live human cells by fluorescence microscopy (Articolo in rivista) (literal)

Anno

• 2014-01-01T00:00:00+01:00 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi

• 10.1016/j.ymeth.2014.01.009 (literal)

Alternative label

• Bergamini C.[ 1 ]; Angelini P.[ 2 ]; Rhoden K.J.[ 1,3 ]; Porcelli A.M.[ 1,2 ]; Fato R.[ 1,2 ]; Zuccheri G.[ 1,2,4,5 ] (2014)
  A practical approach for the detection of DNA nanostructures in single live human cells by fluorescence microscopy
  in Methods (S. Diego Calif. Online)
  (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori

• Bergamini C.[ 1 ]; Angelini P.[ 2 ]; Rhoden K.J.[ 1,3 ]; Porcelli A.M.[ 1,2 ]; Fato R.[ 1,2 ]; Zuccheri G.[ 1,2,4,5 ] (literal)

Pagina inizio

• 185 (literal)

Pagina fine

• 192 (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url

• http://www.scopus.com/inward/record.url?eid=2-s2.0-84899733444&partnerID=q2rCbXpz (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume

• 67 (literal)

Rivista

• Methods (Rivista)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroFascicolo

• 2 (literal)

Note

• Scopu (literal)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni

• [ 1 ] Univ Bologna, Interdept Ctr Ind Res Hlth Sci & Technol CIRI HST, I-40064 Bologna, Italy [ 2 ] Univ Bologna, Dept Pharm & Biotechnol FaBiT, I-40064 Bologna, Italy [ 3 ] Univ Bologna, Dept Med & Surg Sci, I-40064 Bologna, Italy [ 4 ] CNR, Italian Natl Res Council, Ist Nanosci, Ctr S3, Modena, Italy [ 5 ] Univ Bologna, Natl Interuniv Consortium Mat Sci & Technol INSTM, I-40064 Bologna, Italy (literal)

Titolo

• A practical approach for the detection of DNA nanostructures in single live human cells by fluorescence microscopy (literal)

Abstract

• In the last decade, in vivo studies have revealed that even subtle differences in size, concentration of components, cell cycle stage, make the cells in a population respond differently to the same stimulus. In order to characterize such complexity of behavior and shed more light on the functioning and communication amongst cells, researchers are developing strategies to study single live cells in a population. In this paper, we describe the methods to design and prepare DNA-based fluorescent tetrahedral nanostructures, to deliver them to live cells and characterize such cells with epifluorescence microscopy. We report that HeLa cells internalize these nanostructures spontaneously with a higher efficiency with respect to single-stranded or double-stranded oligonucleotides. Our findings suggest that DNA tetrahedra could serve as a platform for the realization of a series of multifunctional intracellular biosensors for the analysis of single live cells. © 2014 Elsevier Inc. (literal)

Prodotto di

• Nanobiosistemi (MD.P06.010.002) (Modulo)

Autore CNR

• Giampaolo Zuccheri (Persona)

Insieme di parole chiave

• Parole chiave di "A practical approach for the detection of DNA nanostructures in single live human cells by fluorescence microscopy" (Insieme di parole chiave)

Incoming links:

Autore CNR di

• Giampaolo Zuccheri (Persona)

Prodotto

• Nanobiosistemi (MD.P06.010.002) (Modulo)

Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#rivistaDi

• Methods (Rivista)

Insieme di parole chiave di

• Parole chiave di "A practical approach for the detection of DNA nanostructures in single live human cells by fluorescence microscopy" (Insieme di parole chiave)