Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers (Articolo in rivista)

Type
Label
  • Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers (Articolo in rivista) (literal)
Anno
  • 2009-01-01T00:00:00+01:00 (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
  • 10.1093/jxb/erp316 (literal)
Alternative label
  • F. Giorno., M.W.-Arts., S. Grillo., Kl. D. Scharf., W. H. Vriezen., and C. Mariani (2009)
    Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers
    in Journal of experimental botany
    (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
  • F. Giorno., M.W.-Arts., S. Grillo., Kl. D. Scharf., W. H. Vriezen., and C. Mariani (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#url
  • http://jxb.oxfordjournals.org/content/61/2/453 (literal)
Rivista
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#note
  • doi:10.1093/jxb/erp316 (literal)
Note
  • ISI Web of Science (WOS) (literal)
Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
  • Department of Plant Cell Biology, IWWR, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands CNR-IGV Institute of Plant Genetics, Via Università 133, 80055 Portici, Naples, Italy Molecular Cell Biology, Goethe University, Max-von-Laue-Str. 9, D-60438 Frankfurt/M., Germany (literal)
Titolo
  • Developmental and heat stress-regulated expression of HsfA2 and small heat shock proteins in tomato anthers (literal)
Abstract
  • The high sensitivity of male reproductive cells to high temperatures may be due to an inadequate heat stress response. The results of a comprehensive expression analysis of HsfA2 and Hsp17-CII, two important members of the heat stress system, in the developing anthers of a heat-tolerant tomato genotype are reported here. A transcriptional analysis at different developmental anther/pollen stages was performed using semi-quantitative and real-time PCR. The messengers were localized using in situ RNA hybridization, and protein accumulation was monitored using immunoblot analysis. Based on the analysis of the gene and protein expression profiles, HsfA2 and Hsp17-CII are finely regulated during anther development and are further induced under both short and prolonged heat stress conditions. These data suggest that HsfA2 may be directly involved in the activation of protection mechanisms in the tomato anther during heat stress and, thereby, may contribute to tomato fruit set under adverse temperatures. (literal)
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