http://www.cnr.it/ontology/cnr/individuo/prodotto/ID28058
Zeolin is a recombinant storage protein that can be used to produce value-added proteins in alfalfa (Medicago sativa L.), (Articolo in rivista)
- Type
- Label
- Zeolin is a recombinant storage protein that can be used to produce value-added proteins in alfalfa (Medicago sativa L.), (Articolo in rivista) (literal)
- Anno
- 2007-01-01T00:00:00+01:00 (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#doi
- 10.1007/s11240-007-9258-8 (literal)
- Alternative label
M.Bellucci F. De Marchis S. Arcioni (2007)
Zeolin is a recombinant storage protein that can be used to produce value-added proteins in alfalfa (Medicago sativa L.),
in Plant cell, tissue and organ culture (Print)
(literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#autori
- M.Bellucci F. De Marchis S. Arcioni (literal)
- Pagina inizio
- Pagina fine
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#numeroVolume
- Rivista
- Note
- ISI Web of Science (WOS) (literal)
- Http://www.cnr.it/ontology/cnr/pubblicazioni.owl#affiliazioni
- Istituto di Genetica Vegetale, Consiglio Nazionale delle Ricerche (CNR), via della Madonna Alta 130, 06128 Perugia, Italy (literal)
- Titolo
- Zeolin is a recombinant storage protein that can be used to produce value-added proteins in alfalfa (Medicago sativa L.), (literal)
- Abstract
- The specific features of plants make them
particularly suitable for the production of recombinant
proteins and alfalfa is one of the recommended
plant production systems. We have transformed
alfalfa with a gene coding for a chimaeric protein
made previously by fusing phaseolin to the Nterminal
region of c-zein and have analyzed the
accumulation of this fusion protein, named zeolin.
Zeolin was expressed both in T0 Regen SY alfalfa
plants and in the progeny resulting from the sexual
cross between Regen SY transformants and alfalfa
cv. Adriana plants. In some alfalfa plants a 95 kDa
zeolin glycosylated polypeptide is the most abundant
polypeptide detected by Western-blot analysis,
whereas in tobacco the most abundant zeolin polypeptide
has a molecular mass around 60 kDa,
expected for intact zeolin. Zeolin has been stably
accumulated in alfalfa leaves because it forms endoplasmic
reticulum-located protein bodies in the cell.
As regards zeolin quantisation, in the progeny alfalfa
plants a value of about 0.22-0.28 mg of zeolin / g of
fresh leaf weight has been estimated. (literal)
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